Share This Author
Improved estimation of secondary structure in ribonucleic acids.
This method can be used for predicting and assessing possible secondary structures for recently determined RNA sequences and new experimental and theoretical results allow us to improve the method, without making it more complicated.
Oligoribonucleotide synthesis using T7 RNA polymerase and synthetic DNA templates.
A method is described to synthesize small RNAs of defined length and sequence using T7 RNA polymerase and templates of synthetic DNA which contain the T7 promoter to increase the variety of RNAs which can be made.
Synthesis of small RNAs using T7 RNA polymerase.
Estimation of Secondary Structure in Ribonucleic Acids
A simple method for estimating the secondary structure of an RNA molecule has been proposed on the basis of the knowledge of its sequence.
Sequence requirements of the hammerhead RNA self-cleavage reaction.
The refined consensus hammerhead resulting from this work was used to identify potential hammerheads present in a variety of Escherichia coli gene sequences, suggesting that the hammerhead contains few, if any, replaceable tertiary interactions as are found in tRNA.
Role of divalent metal ions in the hammerhead RNA cleavage reaction.
Results suggest certain divalent ions either participate directly in the cleavage mechanism or are specifically involved in stabilizing the tertiary structure of the hammerhead.
Uniform Binding of Aminoacyl-tRNAs to Elongation Factor Tu by Thermodynamic Compensation
It is shown that EF-Tu binds misacylated tRNAs over a much wider range of affinities than it binds the corresponding correctly acylated t RNAs, suggesting that the protein exhibits considerable specificity for both the amino acid side chain and the tRNA body.
A kinetic and thermodynamic framework for the hammerhead ribozyme reaction.
A hammerhead ribozyme with eight potential base pairs in each of the substrate recognition helices stabilized product binding sufficiently to enable investigation of the ligation of oligonucleotides bound to the ribo enzymes, providing a basis for future mechanistic studies.
A small catalytic oligoribonucleotide
- O. Uhlenbeck
- Chemistry, BiologyNature
- 1 August 1987
A 19-nucleotide RNA fragment can cause rapid, highly specific cleavage of a 24-nucleotide RNA fragment under physiological conditions. Because each 19-mer can participate in many cleavage reactions,…