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A novel genetic system to detect protein–protein interactions
PROTEIN-protein interactions between two proteins have generally been studied using biochemical techniques such as crosslinking, co-immunoprecipitation and co-fractionation by chromatography. We haveExpand
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An Nα-Acetyltransferase Responsible for Acetylation of the N-terminal Residues of Histones H4 and H2A*
A yeast gene has been identified that encodes a novel, evolutionarily conserved Nα-acetyltransferase responsible for acetylation of the N-terminal residues of histones H4 and H2A. The gene has beenExpand
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An RNA-Binding Protein, hnRNP A1, and a Scaffold Protein, Septin 6, Facilitate Hepatitis C Virus Replication
ABSTRACT Hepatitis C virus (HCV) is a positive-sense single-stranded RNA virus. NS5b is an RNA-dependent RNA polymerase that polymerizes the newly synthesized RNA. HCV likely uses host proteins forExpand
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Translation Initiation on mRNAs Bound by Nuclear Cap-binding Protein Complex CBP80/20 Requires Interaction between CBP80/20-dependent Translation Initiation Factor and Eukaryotic Translation
Background: How the eIF3 complex and ribosomes are recruited during translation on CBP80/20-bound mRNAs remains obscure. Results: CTIF interacts with eIF3g to recruit the eIF3 complex. Conclusion:Expand
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Frequent in-frame length variations are found in the diverged simple repeat sequences of the protein-coding regions of two putative protein kinase genes of Brassica napus
Two putative protein kinase cDNA clones were isolated from Brassica napus by screening with a putative protein kinase cDNA clone of Arabidopsis thaliana. The deduced amino acid sequences show aExpand
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Proline-Rich Transcript in Brain Protein Induces Stress Granule Formation
ABSTRACT The repression of translation in environmentally stressed eukaryotic cells causes the sequestration of translation initiation factors and the 40S ribosomal subunit into discrete cytoplasmicExpand
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Functional complementation of a yeast vesicular transport mutation ypt1-1 by a Brassica napus cDNA clone encoding a small GTP-binding protein
A cDNA clone (bra) encoding a small GTP-binding protein was isolated from Brassica napus by screening a root cDNA library with a degenerate oligonucleotide probe that corresponds to a highlyExpand
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An RNA-Binding Protein , hnRNP A 1 , and a Scaffold Protein , Septin 6 , Facilitate Hepatitis C Virus Replication
Hepatitis C virus (HCV) is a positive-sense single-stranded RNA virus. NS5b is an RNA-dependent RNA polymerase that polymerizes the newly synthesized RNA. HCV likely uses host proteins for itsExpand
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Studies on the Optimal Condition for Ligation of Blunt Ended DNA Fragments
Blunt end DNA ligation의 최적반응조건을 구하기 위하여 반응온도, 완충용액상태, vector와 insert DNA의 농도비율, 반응시간등의 최적조건을 연구하였다. 실험결과 반응최적온도는 $20-25^{\circ}C$ 였고, ATP의 최적농도는 0.5 mM이었다. Spermidine의 농도가 1 mM 인 경우와 4%의 PEG 4,000을Expand
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