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Genomic innovations, transcriptional plasticity and gene loss underlying the evolution and divergence of two highly polyphagous and invasive Helicoverpa pest species
TLDR
The extreme polyphagy of the two heliothines is associated with extensive amplification and neofunctionalisation of genes involved in host finding and use, coupled with versatile transcriptional responses on different hosts. Expand
Proteins Associated with Culex nigripalpus Nucleopolyhedrovirus Occluded Virions
TLDR
Half of the 44 polypeptide sequences identified in this analysis were unique open reading frames (ORFs) encoded by the CuniNPV genome and did not show similarity to any other sequences present in protein databases. Expand
Cloning and expression profiling of odorant‐binding proteins in the tarnished plant bug, Lygus lineolaris
TLDR
Analysis of chemosensory tissues in Lygus lineolaris revealed enrichment of 21 LylinOBP transcripts in antennae, 12 in legs, and 15 in proboscis, suggesting potential roles in olfaction and gustation respectively. Expand
Reduced Levels of Membrane-Bound Alkaline Phosphatase Are Common to Lepidopteran Strains Resistant to Cry Toxins from Bacillus thuringiensis
TLDR
The proteomic and genomic detection of reduced levels of midgut membrane-bound alkaline phosphatase (mALP) as a common feature in strains of Cry-resistant Heliothis virescens, Helicoverpa armigera and Spodoptera frugiperda when compared to susceptible larvae is reported. Expand
Purification, biochemical characterization, and cDNA cloning of a glutathione S-transferase from the red imported fire ant, Solenopsis invicta.
TLDR
Comparison of the deduced amino acid sequence with GSTs from other species revealed that the purified fire ant GST was most closely related to sigma class GSTs. Expand
Cloning and characterization of the Cry1Ac-binding alkaline phosphatase (HvALP) from Heliothis virescens.
TLDR
It is suggested that, depending on glycosylation, all the identified HvmALPs may be synonymous with HvALP, the Cry1Ac-binding phosphatase identified in H. virescens midgut epithelium. Expand
Germ‐line transformation of the South American malaria vector, Anopheles albimanus, with a piggyBac/EGFP transposon vector is routine and highly efficient
TLDR
The piggyBac/PUbnlsEGFP vector appears to be a robust transformation system for this anopheline species, in contrast to the use of a piggy Bac vector in An. Expand
Response of Heliothis virescens (Lepidoptera: Noctuidae) Strains to Bacillus thuringiensis Cry1Ac Incorporated into Different Insect Artificial Diets
TLDR
The data show that Cry1Ac susceptibility was lower in all the H. virescens colonies when challenged with this toxin incorporated into four different insect artificial diets, and highlight the importance of using common diets and use a standard tobacco budworm colony when comparing Cry1ac susceptibility between diverse H.Virescens strains or across time. Expand
Detection of Thelohania solenopsae (Microsporidia: Thelohaniidae) in Solenopsis invicta (Hymenoptera: Formicidae) by multiplex PCR.
TLDR
Multiplex PCR detection of T. solenopsae infection of S. invicta offers the advantages of a positive control, a single PCR amplification, detection of all developmental stages, and increased sensitivity and specificity compared with microscopy. Expand
Identification of the western tarnished plant bug (Lygus hesperus) olfactory co-receptor Orco: expression profile and confirmation of atypical membrane topology.
TLDR
The results suggest that LhOrco likely plays a critical role in mediating L. hesperus odorant perception and discrimination. Expand
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