Genome evolution in yeasts
- B. Dujon, D. Sherman, J. Souciet
- BiologyNature
- 1 July 2004
Analysis of chromosome maps and genome redundancies reveal that the different yeast lineages have evolved through a marked interplay between several distinct molecular mechanisms, including tandem gene repeat formation, segmental duplication, a massive genome duplication and extensive gene loss.
A simple and efficient method for direct gene deletion in Saccharomyces cerevisiae.
- A. Baudin, O. Ozier‐Kalogeropoulos, A. Denouel, F. Lacroute, C. Cullin
- BiologyNucleic Acids Research
- 11 July 1993
A new approach for generating null alleles of a gene by one step PCR amplification of the wild-type HIS3 gene at its own locus is described.
A family of low and high copy replicative, integrative and single‐stranded S. cerevisiae/E. coli shuttle vectors
- N. Bonneaud, O. Ozier‐Kalogeropoulos, Guoya Li, M. Labouesse, L. Minvielle-Sebastia, F. Lacroute
- BiologyYeast
- 1 August 1991
We describe a set of replicative, integrative and single‐stranded shuttle vectors constructed from the pUC19 plasmid that we use routinely in our experiments. They bear a yeast selectable marker:…
Genomic Exploration of the Hemiascomycetous Yeasts: 1. A set of yeast species for molecular evolution studies 1
- J. Souciet, M. Aigle, J. Weissenbach
- BiologyFEBS Letters
- 22 December 2000
Genomic Exploration of the Hemiascomycetous Yeasts: 19. Ascomycetes‐specific genes
- A. Malpertuy, F. Tekaia, B. Dujon
- BiologyFEBS Letters
- 22 December 2000
Functional analysis of YCL09C: Evidence for a role as the regulatory subunit of acetolactate synthase
- C. Cullin, A. Baudin-Baillieu, E. Guillemet, O. Ozier‐Kalogeropoulos
- BiologyYeast
- 1 December 1996
Results show that YCL09C encodes the regulatory subunit of yeast acetolactate synthase, which was previously shown to be retroinhibited by its final product valine.
Purification and characterization of CTP synthetase, the product of the URA7 gene in Saccharomyces cerevisiae.
- W. L. Yang, V. McDonough, O. Ozier‐Kalogeropoulos, M. Adeline, M. Flocco, G. Carman
- BiologyBiochemistry
- 1 September 1994
In the yeast Saccharomyces cerevisiae, CTP synthetase was purified 503-fold to apparent homogeneity from cells bearing the URA7 gene on a multicopy plasmid that directed a 10-fold overproduction of the enzyme.
Genomic Exploration of the Hemiascomycetous Yeasts: 4. The genome of Saccharomyces cerevisiae revisited
- G. Blandin, P. Durrens, B. Dujon
- BiologyFEBS Letters
- 22 December 2000
Random exploration of the Kluyveromyces lactis genome and comparison with that of Saccharomyces cerevisiae.
- O. Ozier‐Kalogeropoulos, A. Malpertuy, J. Boyer, F. Tekaia, B. Dujon
- Biology, EngineeringNucleic Acids Research
- 1 December 1998
In nearly all instances in which the novel K.lactis genes have homologs in different species, sequence conservation is higher with their S.cerevisiae counterparts than with any of the other organisms examined.
Cloning, sequencing and characterization of the Saccharomyces cerevisiae URA7 gene encoding CTP synthetase
- O. Ozier‐Kalogeropoulos, F. Fasiolo, M. Adeline, J. Collin, F. Lacroute
- BiologyMolecular and General Genetics MGG
- 1 December 1991
Gene disruption shows that URA7 is not an essential gene: the level of the intracellular CTP pool is roughly the same in the deleted and the wild-type strains, suggesting that an alternative pathway for CTP synthesis exists in yeast.
...
...