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Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex
Structural-guided engineering of a CRISPR-Cas9 complex to mediate efficient transcriptional activation at endogenous genomic loci is described and the potential of Cas9-based activators as a powerful genetic perturbation technology is demonstrated. Expand
Crystal Structure of Cas9 in Complex with Guide RNA and Target DNA
The crystal structure of Streptococcus pyogenes Cas9 in complex with sgRNA and its target DNA at 2.5 Å resolution and accompanying functional analyses have revealed the molecular mechanism of RNA-guided DNA targeting by Cas9, paving the way for the rational design of new, versatile genome-editing technologies. Expand
Structural Basis for RNA Unwinding by the DEAD-Box Protein Drosophila Vasa
Mutational analyses indicated that the interdomain interactions couple ATP hydrolysis to RNA unwinding, probably through fine positioning of the duplex relative to the wedge helix, which may enable the targeted modulation of intricate RNA structures. Expand
Crystal Structure of the Complex of Human Epidermal Growth Factor and Receptor Extracellular Domains
The crystal structure of a 2:2 complex of human EGF and the EGFR extracellular region has been determined at 3.3 A resolution and the unique "receptor-mediated dimerization" was verified by EGFR mutagenesis. Expand
Crystal structure of the channelrhodopsin light-gated cation channel
The crystal structure of a ChR is presented and the essential molecular architecture of ChRs is revealed, including the retinal-binding pocket and cation conduction pathway, which paves the way for the precise and principled design of ChR variants with novel properties. Expand
Cyclic GMP-AMP as an Endogenous Second Messenger in Innate Immune Signaling by Cytosolic DNA.
Recent progress made in the structural dissection of this cGAS/STING signaling pathway is summarized and possible directions of forthcoming research are indicated. Expand
Crystal Structure of Cpf1 in Complex with Guide RNA and Target DNA
Cpf1 is an RNA-guided endonuclease of a type V CRISPR-Cas system that has been recently harnessed for genome editing. Here, we report the crystal structure of Acidaminococcus sp. Cpf1 (AsCpf1) inExpand
Integrated molecular analysis of adult T cell leukemia/lymphoma
The identified alterations overlap significantly with the HTLV-1 Tax interactome and are highly enriched for T cell receptor–NF-κB signaling, T cell trafficking and other T cell–related pathways as well as immunosurveillance. Expand
Crystal structure of the homologous-pairing domain from the human Rad52 recombinase in the undecameric form.
The crystal structure of the homologous-pairing domain of human Rad52 was determined and revealed that the domain forms an undecameric ring and the amino acid residues located between the beta-beta- beta-alpha fold and the characteristic hairpin loop are essential for ssDNA and dsDNA binding. Expand
Structural basis of Sec-independent membrane protein insertion by YidC
An insertion mechanism for single-spanning membrane proteins, in which the hydrophilic environment generated by the groove recruits the extracellular regions of substrates into the low-dielectric environment of the membrane is proposed. Expand