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Nuclear Pore Complex Structure and Dynamics Revealed by Cryoelectron Tomography
Nuclear pore complexes (NPCs) are gateways for nucleocytoplasmic exchange. To analyze their structure in a close-to-life state, we studied transport-active, intact nuclei from DictyosteliumExpand
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The molecular architecture of lamins in somatic cells
The nuclear lamina is a fundamental constituent of metazoan nuclei. It is composed mainly of lamins, which are intermediate filament proteins that assemble into a filamentous meshwork, bridging theExpand
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Macromolecular Architecture in Eukaryotic Cells Visualized by Cryoelectron Tomography
Electron tomography of vitrified cells is a noninvasive three-dimensional imaging technique that opens up new vistas for exploring the supramolecular organization of the cytoplasm. We applied thisExpand
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Snapshots of nuclear pore complexes in action captured by cryo-electron tomography
Nuclear pore complexes reside in the nuclear envelope of eukaryotic cells and mediate the nucleocytoplasmic exchange of macromolecules. Traffic is regulated by mobile transport receptors that targetExpand
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Outcome of the First Electron Microscopy Validation Task Force Meeting
This Meeting Review describes the proceedings and conclusions from the inaugural meeting of the Electron Microscopy Validation Task Force organized by the Unified Data Resource for 3DEMExpand
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Retrovirus envelope protein complex structure in situ studied by cryo-electron tomography.
We used cryo-electron tomography in conjunction with single-particle averaging techniques to study the structures of frozen-hydrated envelope glycoprotein (Env) complexes on intact Moloney murineExpand
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The human nuclear pore complex as revealed by cryo-electron tomography.
Nuclear pore complexes (NPCs) are the sole passage through the nuclear envelope, connecting the cytoplasm to the nucleoplasm. These gigantic molecular machines, over 100 MDa in molecular weight,Expand
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Protein kinase CK2 and protein kinase D are associated with the COP9 signalosome
The COP9 signalosome (CSN) purified from human erythrocytes possesses kinase activity that phosphoryl ates proteins such as c‐Jun and p53 with consequence for their ubiquitin (Ub)‐dependentExpand
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Functional architecture of the nuclear pore complex.
The nuclear pore complex (NPC) is the sole gateway between the nucleus and the cytoplasm. NPCs fuse the inner and outer nuclear membranes to form aqueous translocation channels that allow the freeExpand
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A progeria mutation reveals functions for lamin A in nuclear assembly, architecture, and chromosome organization
Numerous mutations in the human A-type lamin gene (LMNA) cause the premature aging disease, progeria. Some of these are located in the α-helical central rod domain required for the polymerization ofExpand
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