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Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR
A validated diagnostic workflow for 2019-nCoV is presented, its design relying on close genetic relatedness of 2019- nCoV with SARS coronavirus, making use of synthetic nucleic acid technology.
Guideline to reference gene selection for quantitative real-time PCR.
Today, quantitative real-time PCR is the method of choice for rapid and reliable quantification of mRNA transcription. However, for an exact comparison of mRNA transcription in different samples or
Mutations in the gene encoding the serine protease inhibitor, Kazal type 1 are associated with chronic pancreatitis
Analysis of the gene encoding the serine protease inhibitor, Kazal type 1, a pancreatic trypsin inhibitor, indicates that mutations in SPINK1 are associated with chronic pancreatitis.
Detection of a novel human coronavirus by real-time reverse-transcription polymerase chain reaction.
Two real-time reverse-transcription polymerase chain reaction assays for a novel human coronavirus, targeting regions upstream of the E gene (upE) or within open reading frame (ORF)1b, respectively, observed no cross-reactivity with coronaviruses OC43, NL63, 229E, SARS-CoV or with 92 clinical specimens containing common human respiratory viruses.
Assays for laboratory confirmation of novel human coronavirus (hCoV-EMC) infections.
We present a rigorously validated and highly sensitive confirmatory real-time RT-PCR assay (1A assay) that can be used in combination with the previously reported upE assay. Two additional RT-PCR
Chymotrypsin C (CTRC) variants that diminish activity or secretion are associated with chronic pancreatitis
The results indicate that loss-of-function alterations in CTRC predispose to pancreatitis by diminishing its protective trypsin-degrading activity.
A general method for rapid site-directed mutagenesis using the polymerase chain reaction.
A general and rapid method for site-directed mutagenesis using primed amplification by the polymerase chain reaction to construct mutants of the RNase T1-encoding gene using a double-stranded DNA template.
Improved strategies for sequence-independent amplification and sequencing of viral double-stranded RNA genomes.
This paper reports significant improvements in the efficacy of sequence-independent amplification and quality of sequencing of viruses with segmented double-stranded RNA (dsRNA) genomes and presents the first comparison between the complete consensus sequence of a virulent and an attenuated strain of AHSV1.
CFTR, SPINK1, CTRC and PRSS1 variants in chronic pancreatitis: is the role of mutated CFTR overestimated?
The study demonstrates the complexity of genetic interactions in CP and a minor influence of CFTR alterations in CP development and accumulated CFTR variants are less pronounced than reported previously.
The importance of gene dosage studies: mutational analysis of the parkin gene in early-onset parkinsonism.
This is the first study systematically screening all 12 exons of parkin by real-time, kinetic quantification and clearly shows that mutational analysis of the parkin gene should include gene dosage studies.