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A patch coating method for preparing biocatalytic films of Escherichia coli.
- O. K. Lyngberg, V. Thiagarajan, D. Stemke, J. Schottel, L. Scriven, M. Flickinger
- Biology, EngineeringBiotechnology and bioengineering
- 5 January 1999
Measurements of total RNA and DNA from immobilized and suspended cells indicated that cells immobilized in the thin polymer layer have higher specific beta-galactosidase activity and a slower total RNA degradation rate than suspended cells over 15 days.
Engineering nanoporous bioactive smart coatings containing microorganisms: Fundamentals and emerging applications
Nanoporous, adhesive latex coatings and ink-jet deposited latex microstructures containing concentrated, viable, but nongrowing microorganisms may be useful smart coatings. When rehydrated, these…
A single-use luciferase-based mercury biosensor using Escherichia coli HB101 immobilized in a latex copolymer film
- O. K. Lyngberg, D. Stemke, J. Schottel, M. Flickinger
- BiologyJournal of Industrial Microbiology and…
- 1 July 1999
Patches stored desiccated at room temperature for 2 weeks showed lower mercury-induced luciferase activity when compared to freshly prepared patches, but they still had a considerable detection range of 1 to 10 000 nM HgCl2.
Engineering the Microstructure and Permeability of Thin Multilayer Latex Biocatalytic Coatings Containing E.coli
- O. K. Lyngberg, C. P. Ng, V. Thiagarajan, L. Scriven, M. Flickinger
- Materials ScienceBiotechnology progress
Deff/D decreased by one‐half in approximately 5 days in rehydrated SF091 coatings, indicating that significant wet coalescence occurs after glycerol, sucrose, or trehalose are leached from the films.
Permeability and reactivity of Thermotoga maritima in latex bimodal blend coatings at 80°C: a model high temperature biocatalytic coating
Coating permeability (pore structure) was critical for maintaining T. maritima viability and the observed reactivity and stability of these coatings was surprising since cryo-SEM images suggested that the smaller low Tg polyacrylate/styrene particles preferentially bound to the T. Maritima toga-sheath during coat formation.
Formulation of Reactive Nanostructured Adhesive Microbial Ink-Jet Inks for Miniature Biosensors and Biocatalysis
Reactive adhesive microbial inks can be formulated from aqueous latex emulsions for drop on demand (DOD) piezoelectric deposition. Ink-jet printing of a high density of living microorganisms many be…