SOS chromotest, a direct assay of induction of an SOS function in Escherichia coli K-12 to measure genotoxicity.
- P. Quillardet, O. Huisman, R. D'ari, M. Hofnung
- BiologyProceedings of the National Academy of Sciences…
- 1 October 1982
The results suggest that mutagenic potency measured in the mutatest reflects the level of induction of an SOS function and that most genotoxins are inducers of the SOS response in bacteria.
Cell-division control in Escherichia coli: specific induction of the SOS function SfiA protein is sufficient to block septation.
- O. Huisman, R. D'ari, S. Gottesman
- BiologyProceedings of the National Academy of Sciences…
- 1 July 1984
Induction of the SfiA protein is sufficient to cause inhibition of division in Escherichia coli, and observations reinforce the hypothesis that the sfiB protein, probably required for cell septation, is the target of action of theSfiA division inhibitor.
An inducible DNA replication–cell division coupling mechanism in E. coli
- O. Huisman, R. D'ari
- BiologyNature
- 30 April 1981
The work reported here establishes the existence of the latter type of replication-division coupling in E. coli, and shows that the sfiA gene product is an inducible component of this division inhibition mechanism which is synthesized at high levels after perturbations of DNA replication.
Multiple defects in Escherichia coli mutants lacking HU protein
- O. Huisman, M. Faelen, D. Girard, A. Jaffé, A. Toussaint, J. Rouvière‐Yaniv
- BiologyJournal of Bacteriology
- 1 July 1989
The construction of double mutants totally lacking both subunits of HU protein are reported, which exhibited poor growth and a perturbation of cell division, resulting in the formation of anucleate cells.
Mutational analysis of IS10′s outside end.
- O. Huisman, P. Errada, L. Signon, N. Kleckner
- BiologyEMBO Journal
- 1 July 1989
The phenotypes of mutations in this region suggest that IHF is the major host factor for outside‐end transposition activity in vivo and that base pairs throughout this region are important for the IHF interaction.
Novel mechanism of cell division inhibition associated with the SOS response in Escherichia coli
- R. D'ari, O. Huisman
- BiologyJournal of Bacteriology
- 1 October 1983
Certain Escherichia coli strains were shown to possess a novel system of cell division inhibition, called the SfiC+ phenotype. SfiC+ filamentation had a number of properties similar to those of…
3‐Methyladenine residues in DNA induce the SOS function sfiA in Escherichia coli.
- S. Boiteux, O. Huisman, J. Laval
- BiologyEMBO Journal
- 1 November 1984
It is concluded that the persistence of 3‐methyladenine in E. coli DNA most likely induces the SOS functions and results on in vitro DNA synthesis suggest that this induction is due to an unscheduled arrest of DNA synthesis at this lesion.
A Tn10-lacZ-kanR-URA3 gene fusion transposon for insertion mutagenesis and fusion analysis of yeast and bacterial genes.
- O. Huisman, W. Raymond, M. Hoyt
- BiologyGenetics
- 1 June 1987
A new variant of transposon Tn10 especially adapted for transposition analysis of cloned yeast genes is described, nicknamed mini-Tn10-LUK, which contains a lacZ gene without efficient transcription or translation start signals, an intact URA3 gene, and a kanR determinant.
A new generalizable test for detection of mutations affecting Tn10 transposition.
- O. Huisman, N. Kleckner
- BiologyGenetics
- 1 June 1987
A new rapid screen that allows easy detection of transposon or host mutations that affect Tn10 transposition in Escherichia coli is described and successfully used to identify mutations in the terminal sequences of Tn 10.
Effect of suppressors of SOS-mediated filamentation on sfiA operon expression in Escherichia coli
- O. Huisman, R. D'ari
- BiologyJournal of Bacteriology
- 1 January 1983
The isolation and characterization of sFiA constitutive strains revealed only lexA-linked mutations in a sfiA-background, suggesting that LexA is the only readily eliminated repressor of the sfiC gene.
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