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Small-angle X-ray scattering.
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A new method for the evaluation of small‐angle scattering data
A new numerical method is presented for simultaneous smoothing, desmearing and Fourier transformation of X-ray and neutron small-angle scattering data. The method can only be applied to scattering…
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SAXS experiments on absolute scale with Kratky systems using water as a secondary standard
For small-angle scattering, of X-rays (SAXS) and neutrons (SANS), the importance of absolute calibration has been recognized since the inception of the technique. The work reported here focuses on…
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Structural analysis of porcine brain nitric oxide synthase reveals a role for tetrahydrobiopterin and L‐arginine in the formation of an SDS‐resistant dimer.
TLDR
Low temperature SDS‐polyacrylamide gel electrophoresis revealed that tetrahydrobiopterin and L‐arginine synergistically convert neuronal NOS into an exceptionally stable, non‐covalently linked homodimer surviving in 2% SDS and 5% 2‐mercaptoethanol.
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Characterization and potential applications of nanostructured aqueous dispersions.
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Solving the generalized indirect Fourier transformation (GIFT) by Boltzmann simplex simulated annealing (BSSA)
The structure of colloidal particles can be studied with small-angle X-ray and neutron scattering (SAXS and SANS). In the case of randomly oriented systems, the indirect Fourier transformation (IFT)…
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The interpretation of real-space information from small-angle scattering experiments
The distance distribution function contains all the accessible information about the scattering medium. It is possible to determine the following particle parameters from this function: maximum…
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Adsorption of anionic and cationic surfactants on anionic colloids: supercharging and destabilization.
TLDR
Of particular note is the adsorption of SDS onto the silica nanoparticles, which leads to supercharging of the interface, which has interesting repercussions for structures obtained by the layer-by-layer (LbL) technique, because emulsions stabilized with supercharged and hydrophobized silica are perfect candidates for use in a multilayer system.
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Self-assembled structures and pKa value of oleic acid in systems of biological relevance.
TLDR
Self-assembly of structures in biologically relevant, emulsified oleic acid-monoolein mixtures at various pH values and olei acid concentrations is reported.
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Effect of temperature on self-assembly of bovine beta-casein above and below isoelectric pH. Structural analysis by cryogenic-transmission electron microscopy and small-angle X-ray scattering.
TLDR
It is found that flat disk-like micelles with low aggregation numbers formed at low temperature in the two pH regimes, indicating that below the isoelectric pH (pI), beta-casein loses the distinct separation of hydrophobic and hydrophilic domains, thereby suggesting that it may no longer be considered as a classical head-tail block-copolymer amphiphile as in neutral pH.
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