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SAGA interacting factors confine sub-diffusion of transcribed genes to the nuclear envelope
Changes in the transcriptional state of genes have been correlated with their repositioning within the nuclear space. Tethering reporter genes to the nuclear envelope alone can impose repression andExpand
Nuclear Export of 60S Ribosomal Subunits Depends on Xpo1p and Requires a Nuclear Export Sequence-Containing Factor, Nmd3p, That Associates with the Large Subunit Protein Rpl10p
ABSTRACT Nuclear export of ribosomes requires a subset of nucleoporins and the Ran system, but specific transport factors have not been identified. Using a large subunit reporter (Rpl25p-eGFP), weExpand
Nuclear Retention of Unspliced mRNAs in Yeast Is Mediated by Perinuclear Mlp1
The molecular mechanism underlying the retention of intron-containing mRNAs in the nucleus is not understood. Here, we show that retention of intron-containing mRNAs in yeast is mediated byExpand
Identification of a 60S preribosomal particle that is closely linked to nuclear export.
A nuclear GTPase, Nug1p, was identified in a genetic screen for components linked to 60S ribosomal subunit export. Nug1p cosedimented with nuclear 60S preribosomes and was required for subunit exportExpand
The nucle(ol)ar Tif6p and Efl1p are required for a late cytoplasmic step of ribosome synthesis.
Deletion of elongation factor-like 1 (Efl1p), a cytoplasmic GTPase homologous to the ribosomal translocases EF-G/EF-2, results in nucle(ol)ar pre-rRNA processing and pre-60S subunits export defects.Expand
A Noc Complex Specifically Involved in the Formation and Nuclear Export of Ribosomal 40 S Subunits*
Formation and nuclear export of 60 S pre-ribosomes requires many factors including the heterodimeric Noc1-Noc2 and Noc2-Noc3 complexes. Here, we report another Noc complex with a specific role in 40Expand
High-resolution statistical mapping reveals gene territories in live yeast
The nonrandom positioning of genes inside eukaryotic cell nuclei is implicated in central nuclear functions. However, the spatial organization of the genome remains largely uncharted, owing toExpand
Maturation and Intranuclear Transport of Pre-Ribosomes Requires Noc Proteins
How pre-ribosomes temporally and spatially mature during intranuclear biogenesis is not known. Here, we report three nucleolar proteins, Noc1p to Noc3p, that are required for ribosome maturation andExpand
Hmo1, an HMG‐box protein, belongs to the yeast ribosomal DNA transcription system
Hmo1 is one of seven HMG‐box proteins of Saccharo myces cerevisiae. Null mutants have a limited effect on growth. Hmo1 overexpression suppresses rpa49‐Δ mutants lacking Rpa49, a non‐essential butExpand
High-throughput chromatin motion tracking in living yeast reveals the flexibility of the fiber throughout the genome.
Chromosome dynamics are recognized to be intimately linked to genomic transactions, yet the physical principles governing spatial fluctuations of chromatin are still a matter of debate. UsingExpand