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Bacterial β-Kdo glycosyltransferases represent a new glycosyltransferase family (GT99)
TLDR
The crystallographic structure and function of a prototype β-Kdo GT from WbbB, a modular protein participating in LPS O-antigen synthesis in Raoultella terrigena is reported, suggesting an evolutionary connection that is not immediately evident from the overall fold or sequence similarities. Expand
Unique lipid anchor attaches Vi antigen capsule to the surface of Salmonella enterica serovar Typhi
TLDR
This work identifies a reducing terminal lipid structure unique to the Vi antigen that is required for attachment of the capsular surface layer of Vi antigen, and has potential implications in pathogenesis and vaccine production. Expand
Molecular basis for the structural diversity in serogroup O2-antigen polysaccharides in Klebsiella pneumoniae
TLDR
Two new loci encoding enzymes that modify a conserved polysaccharide backbone comprising disaccharide repeat units are reported, providing a complete understanding of the molecular genetic basis for the known variations in Klebsiella O-antigen carbohydrate structures based on the O2a backbone. Expand
Structure and genetic basis of Yersinia similis serotype O:9 O-specific polysaccharide
TLDR
The characterization of the OPS structure and gene cluster of Y. similis O:9 OPS resembles none of the receptor structures of the Y. enterocolitica strains, suggesting that φR1-37 can recognize several surface receptors, thus promoting broad host specificity. Expand
Musculature of the ovipositor of Lenitovena trigona (Matsumura) (Diptera, Tephritidae) with reference to the larval saprophagy
TLDR
The structure of the sclerites and musculature of the ovipositor of Lenitovena trigona (Matsumura) a Far Eastern tephritid of the tribe Acanthonevrini with initially saprobiont larva was investigated to facilitate understanding of the morphological changes in the adults associated with larval shift from saprophagous to herbivorous habit. Expand
Variations in O-Antigen Biosynthesis and O-Acetylation Associated with Altered Phage Sensitivity in Escherichia coli 4s
ABSTRACT The O polysaccharide of the lipopolysaccharide (O antigen) of Gram-negative bacteria often serves as a receptor for bacteriophages that can make the phage dependent on a given O-antigenExpand
Localization and molecular characterization of putative O antigen gene clusters of Providencia species.
TLDR
The gene functions predicted in silico were in agreement with the known O antigen structures; furthermore, annotation of the genes involved in the three-step synthesis of GDP-colitose (gmd, colD and colC) was supported by cloning and biochemical characterization of the corresponding enzymes. Expand
Cold Stress Makes Escherichia coli Susceptible to Glycopeptide Antibiotics by Altering Outer Membrane Integrity.
TLDR
It is demonstrated that the Gram-negative bacterium Escherichia coli becomes susceptible to the narrow-spectrum antibiotic vancomycin during growth at low temperatures, and unexpected observations challenge the current understanding of outer membrane permeability, and provide new chemical insights into the susceptibility of E. coli to glycopeptide antibiotics. Expand
Single polysaccharide assembly protein that integrates polymerization, termination, and chain-length quality control
TLDR
Raoultella terrigena WbbB is described, a prototype for a family of proteins that, remarkably, integrates several key activities in polysaccharide biosynthesis into a single polypeptide. Expand
Biochemical Characterization of Bifunctional 3-Deoxy-β-d-manno-oct-2-ulosonic Acid (β-Kdo) Transferase KpsC from Escherichia coli Involved in Capsule Biosynthesis*
TLDR
The linkage specificities of the glycosyltransferase domains are proposed to be conserved in KpsC homologs from other bacterial species, based on phylogenetic analyses. Expand
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