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Capillary electrophoresis with LED-induced native fluorescence detection for determination of isoquinoline alkaloids and their cytotoxicity in extracts of Chelidonium majus L.
The developed method was successfully applied to determine the contents of seven alkaloids in the aerial parts of Chelidonium majus L, which varied from 0.025 to 0.763% (w/w), and an estimation of the cytotoxic properties of selected Celandine alkaloid in a natural extract was carried out. Expand
Smoothened agonist augments proliferation and survival of neural cells
In vitro experiments demonstrated that SAG in low-nanomolar concentrations induces proliferation of neuronal and glial precursors without affecting the differentiation pattern of newly produced cells and, in contrast to Shh, SAG did not induce neurotoxicity in neuronal cultures. Expand
In situ fibrillizing amyloid-beta 1-42 induces neurite degeneration and apoptosis of differentiated SH-SY5Y cells
It is shown that differentiated neuron-like SH-SY5Y cells are more sensitive to amyloid peptides than non-differentiated cells, because the latter lack long neurites, which support the hypothesis that peptide toxicity is related to the active fibrillization process in the incubation mixture. Expand
Mucin5B expression by lung alveolar macrophages is increased in long‐term smokers
M UC5B is expressed in a subset of lung AMs and long‐term cigarette smoking may increase the level of MUC5B produced by these cells. Expand
Sonic Hedgehog pathway activity in prostate cancer.
A significant age-dependent increase in the number of Shh, Gli1,gli3 and FoxA1-positive prostate cells and a decrease in Gli2-positive cells in TRAMP is shown, which supports the hypothesis that the development of prostate cancer and its metastasis is associated with activation of the Shh signaling pathway. Expand
Glucose up‐regulates HIF‐1α expression in primary cortical neurons in response to hypoxia through maintaining cellular redox status
Evidence is presented that glucose promoted HIF‐1α stabilization through regulating redox status in primary neurons exposed to hypoxia, and that a reducing environment is required to stabilize HIF-1α in neurons exposure to Hypoxia. Expand
Copper(I)-binding properties of de-coppering drugs for the treatment of Wilson disease. α-Lipoic acid as a potential anti-copper agent
It is hypothesized that the endogenous biologically active substance, α-lipoic acid, may be suitable for the treatment of Wilson disease, and the results provide a basis for elaboration of new generation drugs that may provide better therapeutic outcomes. Expand
Generation and characterization of mouse monoclonal antibody 5E1 against human transcription factor GLI3.
The production of mouse monoclonal antibody (MAb) against putative repressive motif in GLI3 (GLI3pRM) showed that MAb 5E1 was immunoglobulin IgM/ê and it recognized recombinant human GLI 3pRM specifically. Expand
Capillary electrophoretic study of the synergistic biological effects of alkaloids from Chelidonium majus L. in normal and cancer cells
  • M. Kulp, O. Bragina
  • Chemistry, Medicine
  • Analytical and Bioanalytical Chemistry
  • 29 January 2013
The results showed a differential ability of celandine alkaloids to penetrate into the normal and cancer cell interior, which was inversely proportional to their cytotoxic activity. Expand
Metallothionein 2A affects the cell respiration by suppressing the expression of mitochondrial protein cytochrome c oxidase subunit II
It is demonstrated that overexpression of MT-2A in HEK cell line decreased the oxidative phosphorylation capacity of the cells and reduced oxygen consumption and lower cellular ATP levels. Expand