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Effect of benzyladenine on recovery of cryopreserved shoot tips of grapevine and citrus cultured in vitro.
TLDR
Results from the present study suggest that an optimal BA concentration for recovery of control shoot tips may be different from that for cryopreserved shoot tips; furthermore, the optimal BA concentrations may also differ among different cryogenic procedures. Expand
Two classes of subgenomic RNA of grapevine virus A produced by internal controller elements.
TLDR
This work explored the production of viral RNAs in a GVA-infected Nicotiana benthamiana herbaceous host and characterized one nested set of three 5'-terminal sgRNAs, which were abundant in dsRNA-enriched extracts and suggested that each of these cis-acting elements is involved in production of four RNAs. Expand
Role of the Insect Supervectors Bemisia tabaci and Frankliniella occidentalis in the Emergence and Global Spread of Plant Viruses.
TLDR
The plant virus-supervector interaction offers exciting opportunities for basic research and global implementation of generalized disease management strategies to reduce economic and environmental impacts. Expand
Transgenes consisting of a dsRNA of an RNAi suppressor plus the 3′ UTR provide resistance to Citrus tristeza virus sequences in Nicotiana benthamiana but not in citrus
TLDR
None of the plants showed durable resistance to Citrus tristeza virus, as indicated by the obtaining of similar Northern hybridization signals from both transgenic and non-transgenic citrus plants. Expand
Engineering the genome of Grapevine virus A into a vector for expression of proteins in herbaceous plants.
TLDR
The genome of GVA was engineered as a vector that includes duplication of homologous sequences that contain the promoter of the movement protein (MP) sgRNA, supplemented by enzymatic restriction sites to be used as a convenient tool for transient expression of foreign genes from an individual sg RNA. Expand
Tomato chocolate spot virus, a member of a new torradovirus species that causes a necrosis-associated disease of tomato in Guatemala
TLDR
Analysis of nucleic acids associated with purified virions of the chocolate-spot-associated virus revealed a genome composed of two single-stranded RNAs similar to recently described torradoviruses, a new group of picorna-like viruses causing necrosis-associated diseases of tomatoes in Europe and Mexico. Expand
An Outbreak of a Necrosis Disease of Tomato in California in 2008 was Caused by a New Ilarvirus Species Related to Parietaria mottle virus.
TLDR
Results indicate the necrosis disease of tomato is caused by a new ilarvirus species, tentatively named Tomato necrotic spot virus, although further studies are needed to confirm this. Expand
First Report of Tomato chlorotic spot virus in Processing Tomatoes in the Dominican Republic.
TLDR
TCSV is causing the tospovirus disease of processing tomato, and this is the first report of this virus in rogostrips in the Dominican Republic. Expand
Construction of a multiprobe for the simultaneous detection of viroids infecting citrus trees
TLDR
The construction of a CVd-Multiprobe consisting of full-length clones of Hopstunt viroid, Citrus exocortis viroid and Citrus bent leaf viroid is described, which does not provide information on the specific viroid resulting in a positive signal. Expand
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