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Multiplex PCR for the detection of African cassava mosaic virus and East African cassava mosaic Cameroon virus in cassava.
The multiplex PCR is useful for reliable assessment of the prevalence of CMBs in epidemiological studies and for crop improvement and phytosanitary programs in African countries. Expand
A Leafhopper-Transmissible DNA Virus with Novel Evolutionary Lineage in the Family Geminiviridae Implicated in Grapevine Redleaf Disease by Next-Generation Sequencing
Molecular and phylogenetic analyses indicated that GRLaV, almost identical to recently reported Grapevine Cabernet Franc-associated virus from New York and Grapevine red blotch- associated virus from California, represents an evolutionarily distinct lineage in the family Geminiviridae with genome characteristics distinct from other leafhopper-transmitted geminivIRuses. Expand
Banana bunchy top virus in sub-Saharan Africa: investigations on virus distribution and diversity.
Results imply that farmers' traditional practice of transferring vegetative propagules within and between countries, together with virus spread by the widely prevalent banana aphid vector, Pentalonia nigronervosa, could have contributed to the geographic expansion of BBTV in SSA. Expand
Grapevine leafroll-associated virus 1 occurs as genetically diverse populations.
The genetic landscape of GLRaV-1 populations presented in this study provides a foundation for better understanding of the epidemiology of grapevine leafroll disease across grape-growing regions in the United States. Expand
Status of Cassava Begomoviruses and Their New Natural Hosts in Nigeria.
Although the virulent Ugandan variant of East African cassava mosaic virus (EACMV-Ug2) was not detected, the occurrence of variants of ACMv and a high proportion of mixed infections by ACMV and EACV, which could result in recombination events such as the one that produced EACMVs, demands appropriate measures to safeguard cassava production in Nigeria. Expand
Genetic variability of natural populations of Grapevine leafroll-associated virus 2 in Pacific Northwest vineyards.
Genetic variability of field populations of Grapevine leafroll-associated virus 2 (GLRaV-2) in Pacific Northwest (PNW) vineyards was characterized by sequencing the entire coat protein and a portion of the heat-shock protein-70 homolog (HSP70h) genes, which indicated different selection pressures may be acting on the two genomic regions encoding proteins with distinct functions. Expand
Alternate hosts of African cassava mosaic virus and East African cassava mosaic Cameroon virus in Nigeria
Cassava mosaic disease (CMD) caused by African cassava mosaic virus (ACMV) and East African cassava mosaic Cameroon virus (EACMCV) is the major constraint to cassava production in Nigeria. SequencesExpand
Sequence diversity, population genetics and potential recombination events in grapevine rupestris stem pitting-associated virus in Pacific North-West vineyards.
The discordant gene phylogenies obtained for some CP and HR sequences and the identification of potential recombination events involving parents from different lineages provided strong evolutionary evidence for genetic diversity among GRSPaV isolates, underscoring the highly variable nature of the virus. Expand
Description of a Novel Monopartite Geminivirus and Its Defective Subviral Genome in Grapevine.
A novel virus was detected in grapevines by Illumina sequencing during the screening of two table grape accessions, cultivars Black Beet and Nagano Purple, from South Korea, and placed in a unique taxon within the family Geminiviridae. Expand