O. V. Payushina

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Fetal liver, during its hematopoietic activity, contains mesenchymal stromal cells (MSCs) generating its hematopoietic microenvironment. These cells are clonogenic and capable of multilineage differentiation; however, little is known about how their properties alter during embryogenesis. We compared the cloning efficiency of MSCs from rat fetal liver at 14,(More)
394 Studies on mesenchymal stromal cells (MSCs) are a focus of modern biology. Initially, MSCs were isoo lated from the bone marrow, spleen, and thymus in the form of colonyyforming units of fibroblasts (CFUUF) [1]; later, MSCs were found in all organs of transient and definitive hematopoiesis. Correlation between the contents of CFUUF and hematopoietic(More)
It is shown that the effect of 5-fluorouracil (5-FU) on mesenchymal stromal cells (MSCs) of the bone marrow and fetal liver of the rat enriches the population with cells with a reduced proliferative potential. It is shown that, in the course of passaging, the 5-FU-resistant MSCs of the bone marrow and fetal liver lose their ability to differentiate in the(More)
Hematopoiesis occurs in a specific microenvironment formed by the stroma of hematopoietic organs characterized by variable cellular composition. The combined action of stromal elements controls the proliferation and differentiation of hematopoietic cells by means of direct cell-cell interactions and the production of humoral factors. The role of different(More)
Comparison of mesenchymal stromal cells of embryonic and adult rat spleen showed that splenic cells from 20-day rat fetuses exhibit the capacity for clonal growth, express surface antigens CD73, CD90, and CD106, and have weak osteogenic and adipogenic potencies, while splenic cells from adult animals are characterized by lower cloning efficiency, rapid(More)
Permanent embryonic stem cell lines (ES cells) are considered as one of the most promising cellular sources for regenerative medicine. ES cells have a high proliferative potency and ability to differentiate into all kinds of somatic and germ cells. However, transplantation of undifferentiated ES cells into adult recipient tissue results in the formation of(More)
The study of mesenchymal stromal cells (MSCs) is a very topical problem. Numerous experiments in vitro advanced the understanding of MSC biology to a great extent. However, many aspects of their behavior in vivo still remain unclear. This review deals with MSC localization and functioning in an organism. MSCs are present in various tissues, changing their(More)
Fetal liver is known as a source of multipotent mesenchymal stromal cells. These cells are routinely isolated by adhesion to plastic, but thus prepared culture is contaminated by other cells. For instance, primary cell culture of from rat fetal liver, apart from fibroblasts with phenotypic characteristics of mesenchymal stromal cells, contained skeletal(More)
The molecular basis of development and regeneration of skeletal muscles are reviewed. A model of parent-progeny relationships of mature animals’ skeletal muscles is proposed. Different cellular populations that contribute to myogenesis in vivo and in vitro are described. Both well-known typical cellular sources for muscle regeneration (satellite cells,(More)
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