O. S. Kuprienko

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Diethylenetriaminetetraacetic acid-based bifunctional reagents containing a bound Eu3+ ion and a reactive group capable of interacting with proteins or low-molecular-weight compounds have been synthesized. These reagents are intended for use in lanthanide immunofluorimetric assay with a dissociation-enhancing solution (DELFIA). A complexonate of Eu3+ and N(More)
Bifunctional reagents based on diethylenetriaminetetraacetic acid containing a bound metal ion and a reactive functional group for the interaction with proteins and low-molecular-weight substances have been synthesized. An Amino-derivative of a complexonate was obtained by acylation of monosubstituted diamine with diethylenetriaminepentaacetic acid(More)
This paper describes the in vivo generation method of biotinylated recombinant Fab antibody fragments. The original molecular vectors for Escherichia coli Fab fragments expression were designed. In vivo biotinylated recombinant antibody Fab fragment against cortisol was generated. The kinetic parameters of interaction of these antibody fragments with(More)
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