O N Kniagina

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A method is suggested for the detection of the human rotavirus VP4 gene by RT-PCR. The method is universal for all [P] types of group A rotavirus in clinical samples. As against RNA electrophoresis and ELISA, it provides for a more accurate detection of rotaviruses by 18.8 and 26.5%, respectively. It was established by RT-PCR in February, 2002, in Nizhny(More)
The RT-PCR assay for the detection of all human enteroviruses was used to analyze fecal samples, cerebrospinal fluid (CSF), respiratory tract swabs, and sera from 204 patients with symptoms of viral infection. Enteroviruses were detected in 31.37% of cases. The proposed method analyzes every type of clinical samples. The relative detection rate of(More)
AIM Analysis of manifestation of epidemic process of aseptic meningitis and causes of its activation using molecular genetic methods. MATERIALS AND METHODS Samples of feces and CSF, nasopharyngeal swabs from 296 patients with aseptic meningitis (AM), as well as 240 samples of drinking water and 6 samples of lake water were studied. Epidemiologic analysis,(More)
41 children with chronic renal diseases (with different forms of chronic glomerulonephritis or chronic renal failure) previously vaccinated with 5 to 9 doses of oral poliovaccine (OPV) were examined. Analysis of immunity to polioviruses revealed insufficient serologic protection against all three types of polioviruses compared with general population.(More)
AIM To assess immunization coverage against poliomyelitis and level of immunity postvaccination in children with type 1 diabetes mellitus (DM1) and rheumatic diseases. MATERIALS AND METHODS Vaccination status of 299 children with DM1 and 136 children with rheumatic diseases was determined. Serologic test using neutralization reaction was performed in 31(More)
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