O. L. Zaika

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A fluorescent probe, FURA-2M, was used to examine the role of mitochondria in the generation of calcium transients evoked by acetylcholine (ACh) in isolated rat chromaffin cells. Our experiments showed that application of 10 μM CCCP (carbonyl cyanide m-chlorophenylhydrazone, a mitochondrial protonophore) caused significant intracellular calcium transients(More)
We studied the role of one of the synaptic proteins belonging to the SM-protein family, Munc18, in the process of exocytosis. For this purpose, we used microinjections of antibodies against protein Munc18 (anti- Munc18) into isolated chromaffin cells of the rat adrenal gland, with the aim of suppressing the function of this protein. Secretion of(More)
Activation of acetylcholine receptors (AChR) triggers catecholamine release from adrenal chromaffin cells and release of neurotransmitters in neuron-to-neuron and neuromuscular junctions, including those on smooth muscle cells. Calcium ions play the role of the main intracellular messenger, which mediates these processes. In our study, we explored the(More)
A recently developed technique for electrochemical identification opens new prospects for stydying transmitter release from single cells. Using an express cyclic voltammetric technique, we studied the properties of two neurontransmitters, serotonin and dopamine, which can be simultaneously released in the course of synaptic transmission. We showed that both(More)
We used an intracellular fluorescent probe, FURA-2M, to examine the responses of isolated rat chromaffin cells to applications of 1 mM acetylcholine (ACh). Our data showed two different populations of the cell responses to such stimulation. Responses of the first type demonstrated fast rise and decay phases of the Ca2+ transients and no significant decrease(More)
Influence of agents inducing secretion: KCl-induced membrane depolarization and acetylcholine on catecholamine secretion from individual vesicles of rat chromaffin cells was studied by using amperometric methods. It has been shown that secretory components participating in these processes are different. An importance of cellular response on different kind(More)
We studied the involvement of the endoplasmic reticulum (ER) in calcium signaling in rat chromaffin cells. For this purpose, the following agents influencing the activity of the ER were used: (i) Caffeine that activates the release of Ca2+ from the endoplasmic store and (ii) thapsigargin that suppresses accumulation of calcium in the ER. The intracellular(More)
The molecular mechanisms of secretion in chromaffin cells possess many features similar to those of exocytotic neurotransmitter release in the synapses. It should be taken into account that chromaffin cells are formed from the precursor cells common to these units and neuronal elements. Numerous studies of the intracellular mechanisms of exocytosis showed(More)
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