O. J. L. Oud

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Using fluorescence in situ hybridization (FISH) with metaphase preparations, we localized a 4-kb single-copy T-DNA sequence in a group of petunia transformants. The selected T-DNAs previously had been shown to be linked to the phenotypic marker FI on chromosome II. Linkage analysis had revealed that recombination around the FI locus is suppressed in a wide(More)
In an earlier fluorescent in-situ hybridization (FISH) study on petunia (ten Hoopen et al. 1996), we found a considerable discrepancy between the genetic map and the physical map with respect to T-DNA insertions on metaphase chromosomes. For some transgenes we found a preference to integrate near the telomeres. Here, we studied the spatial position of(More)
 Chromosomes elongated beyond a critical size by balanced rearrangements reduce the viability and fertility of field bean individuals. The severity of symptoms, ranging from growth retardation to early death of seedlings, increases with the length of the longest chromosome arm. This is paralleled by the incomplete separation of sister chromatids during(More)
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