O Iu Pletiushkina

Learn More
P66shc protein is an alternative transcript product of SHC1 gene. While two other isoforms (p52shc and p46shc) have adaptor function in RAS signaling pathway, p66shc regulates reactive oxygen species (ROS) level. P66shc genome knockout significantly extends lifespan in mice. Though p66shc was determined to translocate into mitochondria and led to increase(More)
Mouse embryonic fibroblasts (MEF) with point mutation in somatic cytochrome C gene were generated and characterized. It was shown that substitution of lysine for tryptophan in position 72 (K72W) decreased the proapoptotic functions of cytochrome C in response to staurosporin treatment without disrupting its respiratory functions. The presence of this(More)
Normal mouse fibroblasts are spread over the glass in the cultural medium containing cytochalasin B (CB). The cultures were incubated with concanavalin A (Con A), which binds some surface components. Con A-labeled surface receptors were visualized by the indirect immunogluorescent method. When living cultures were incubated with Con A, small patches of Con(More)
Stationary cultures of the mouse transformed cells L and S-40 sensitive to topoinhibition were found to be insensitive to the action of hyaluronidase, RNAase, and colcemid in doses known to stimulate multiplication of normal mouse fibroblasts. These cultures were still insensitive to the action of medium change and removal of a part of the monolayer.
A tumor promoter phorbol 12-myristate 13-acetate (PMA) induces characteristic reversible changes in the cell shape in certain fibroblastic lines. This reaction to PMA may be regarded as a prototype of reorganizations involving formation of stable cytoplasmic processes. Two specific drugs, Taxol and Colcemid, were used to study the role of microtubules and(More)
Substrate-attached normal mouse fibroblasts, transformed mouse fibroblasts (L-strain) and epithelial cells (MPTR strain) were incubated with two ligands that are cross-linking different group of the surface receptors: concanavalin A and cationic ferritin. Surface-attached ligands were revealed by the indirect immunofluorescent methods. The incubation of(More)
Normal mouse fibroblasts, transformed mouse fibroblasts (L strain), the ascite hepatoma cells (strain 22), the Krebs II ascite cells, and mouse spleen lymphocytes were incubated in suspension in the medium containing concanavalin A which is capable of crosslinking definite groups of surface receptors. Surface-attached ligands were revealed by both direct(More)
Small fragments of the peripheral cytoplasm were obtained from cytochalasine B-treated mouse embryo fibroblasts and studied for distribution of microtubules by indirect immunofluorescence. Microtubules were demonstrated to progressively depolymerize in these fragments which did not contain any tubules after 6 hours of incubation in the growth medium. This(More)