O. A. Vorob'eva

Learn More
Using radioimmunological method, the level and dynamics of thyroid hormones, thyroxine (T4) and triiodothyronine (T3) were studied in eggs, embryos, and pre-larvae of the Russian sturgeon Acipenser güldenstadti L. The level of hormones in eggs was higher than in the individuals at the stage of hatching. In embryogenesis the content of T4 and T3 hormones(More)
The role of some intraovarian regulators of the final stages of gametogenesis is analysed. It is shown that the epidermal growth factor (EGF) in concentration of 1 and 10 ng/ml is able to induce reinitiation of meiosis from dictyotene stage during cultivation of the ovarian follicles of prepuberal mice in the serum-free medium after gonadotrophic(More)
A method of obtaining granulosa cell culture reacting to the action of gonadotropins and growth factors is described. The efficiency of cell cloning is enhanced under influence of insulin, epidermal growth factor (EGF) and fibroblast growth factors (FGF). Stimulation of proliferation by the latter two factors is seen in the medium with the low serum(More)
Experimental data on the role of growth factors in the processes of oocyte maturation and of proliferation and differentiation in the mammalian ovaries are reviewed. Evidence is provided that theca and granulosa cells can synthesize epidermal, fibroblast, insulin-like and transforming growth factors and have receptors to all of them. The regulation of(More)
Human oocytes that failed to be fertilized in vitro were fixed for cytogenetic analysis. Successful karyotyping was achieved in 157 oocytes. Among women aged 23-35 years, 34% of oocytes were aneuploid and 8.6% diploid, while for women aged 36-39 years, these values exceeded 41 and 11.8%, respectively. The proportion of aneuploid and diploid oocytes(More)
This study was initiated to determine the relationship between the fertilizing potential of spermatozoa and abnormalities in the compact packing of their chromatin which occurs in the final stage of male germ cell differentiation. Chromatin packing involves disulphide bridge covalent cross-linking. The degree of packing was determined from the accessibility(More)
The features of steroidogenesis of immature mouse ovaries in culture under the influence of follicle-stimulating hormones (FSH), human chorionic gonadotropin (hCG), epidermal growth factor (EGF) and insulin have been investigated during the period of reinitiation of meiosis in the oocytes. Secretion of progesterone is stimulated after addition of FSH, hCG(More)
The study has evaluated 304 cases under of vitro fertilization. Positive correlations were shown of semen parameters and fertilization rate. The individual spectrum of different sperm morphological types was shown to be constant. The discriminant analysis was used for comparison of two groups of patients--with low (less than 33%) and high (more than 67%)(More)