O. A. Toropygina

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Chromatophores and peripheral light-harvesting complexes B800–850 with a trace of carotenoids were isolated from Chromatium minutissimum cells in which carotenoid biosynthesis was inhibited by diphenylamine. Three methods previously used for the reconstitution of carotenoids into either the light-harvesting (LH1) type complexes or reaction centers (RC) of(More)
The spatial relationship of polypeptides comprising the light-harvesting B800-850 complex of Chromatium minutissimum has been studied by means of chemical crosslinking of the isolated complex with cleavable, 1.2 nm-long dithiobis(succinimidyl propionate). The samples were analyzed by different types of electrophoresis and spectrophotometrically. No(More)
Okenone was reconstituted into light harvesting (LH) complexes of the purple photosynthetic bacterium Allochromatium minutissimum possessing the spirilloxanthin pathway for carotenoid biosynthesis. Suppression of this pathway by diphenylamine, an inhibitor of carotenogenesis, yielded nearly carotenoidless complexes preserving their native spectral(More)
Two-photon fluorescence excitation spectra of the peripheral light-harvesting complex LH2 from the purple photosynthetic bacterium Chromatium minutissimum were examined within the expected spectral range of the optically forbidden S1 singlet state of carotenoids. LH2 preparations isolated from wild-type and carotenoid-depleted cells were used. 100-fs laser(More)
Pigment–protein complexes in the photosynthetic membranes of purple bacteria fall into three major types: antenna light-harvesting complexes LH1 (B880), antenna light-harvesting complexes LH2 (B800–850), and photosynthetic reaction centers (RC) [1]. Antenna complexes are composed of two lowmolecular-weight subunits ( α and β ). The polypeptide chain of each(More)
Andrey Moskalenko, Olga Toropygina and Nina Kuznetsova Institute of Soil Science and Photosynthesis RAS, Pushchino, 142292, Russia Z. Naturforsch. 51c, 309-318 (1996); received D ecem ber 12, 1995/February 8, 1996 Purple Bacteria, Light-Harvesting Complex, B880, B820, D issociation, Transform ation, C arotenoid A study is reported on the modification of the(More)
Treatment of some sulfur bacteria (Allochromatium minutissimum, Thiorhodospira sibirica, and Ectothiorhodospira halovacuolata WN22) with dioxane results in formation of the bacteriochlorophyll form B820 in the light harvesting complex LH2. This form characterized by absorption maximum at 820 nm has the same absorption spectrum as B820 subcomplex from LH1(More)
According to the generally accepted view, carotenoids play several important roles in photosynthesis, including light-harvesting, protective, and structural functions [1, 2]. The goal of this work was to analyze the protective function of carotenoids in more detail. For the first time, the possible protective role of carotenoids in photosynthesis was(More)
Spatial relationships between different pigment-protein complexes in the membranes of the purple photosynthetic bacterium, Chromatium minutissimum, have been studied. The possibility of restoring the function of efficient excitation energy transfer from bacteriochlorophyll molecules to the reaction centers in the system of soybean liposomes, reconstituted(More)
A simple method of revealing the additional zones of proteins in gradient polyacrylamide gels, preliminary dyed Coomassie by means of silver ions is described. The dyeing of Coomassie allows to avoid the time-consuming stages of preliminary treatment of gels as well to reveal more sensitive zones in gels. On the second stage of dyeing silver minor zones(More)
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