O. A. Strel'chenok

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A biochemical system of radioimmunoassay of human thyroid peroxidase (TPO) with the use of specific autoantibodies was developed for the first time. This system includes lyophilized preparations of human autoantibodies to TPO, radioiodinated TPO, standards prepared from pure TPO, and the solid-phase protein A as a precipitating agent. An analytical system(More)
Amides of all trans-retinoic acid and O-phospho-L-threonine, O-phospho-L-tyrosine, and O-phosphoethanolamine injected intravenously in a dose of 6.8 μg/kg 1.5-3.4-fold increased the count of antibody-producing cells in the spleen of C57Bl/6 mice (primary immune response to sheep erythrocytes). Activity of the complex containing α-fetoprotein and L-threonine(More)
A methodological approach to development of a radioimmunological system for quantitative assay of thyroxine (T4) in human blood serum is described. It implies investigation of every component of the system and matching of the components by the physico-chemical properties and concentrations. An antiserum to the T4 conjugate was prepared with bovine serum(More)
It was demonstrated that transcortin complexed with progesterone specifically binds to plasma membranes of human decidual endometrium, a progesterone target tissue. This interaction is characterized by a high affinity [Kd = (1.0 +/- 0.2).10(-10) mol/l] and selectivity. Such human serum proteins as albumin, orosomucoid, transferrin, thyroxine- and sex(More)
The data on interaction of specific steroid-binding glycoproteins of blood plasma and of their steroid-hormone complexes with the plasma membranes of various hormone target tissues suggests that these glycoproteins play an active role in the transport of steroid hormones into the target cells. Both polypeptide and carbohydrate components of the(More)
Interactions of transcortin (corticosteroid-binding globulin, CBG) variants, nCBG and rCBG, present in the blood of pregnant women, and microvesicular fraction of the brush border membrane of human placental syncytiotrophoblast at 23 +/- 2 degrees C were studied. Interaction of nCBG in complex with a steroid with each of the two types for specific binding(More)
Retinoids are known to possess a broad spectrum of biological activity, which is related to the ability of these compounds to interact with RAR and RXR – the intrisic receptors containing transcription factors belonging to the steroid – thyroid superfamily of nuclear hormonal receptors [1]. Exhibiting strong antiproliferative and cytodifferentiating(More)
The effects of steroids on specific binding of transcortin (corticosteroid-binding globulin, CBG) variants (nCBG and vCBG) from pregnant women sera to the microvesicular membrane fraction derived from placental syncytiotrophoblast brush border have been studied. It was shown that native CBG variants stripped of steroids lose their ability to interact with(More)