Nurit Davidovich

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Simulating ventilator-induced lung injury (VILI) in the laboratory requires stretching of lung alveolar tissue. Whereas precision-cut lung slices (PCLSs) are widely used for studying paracrine signaling pathways in the lungs, their use in stretch studies is very limited because of the technical challenge of fixing them to a stretchable substrate, stretching(More)
Human lung tissue donated for research purposes is a precious resource which can enhance the exploration of mechanisms involved in ventilator-induced lung injury (VILI). The goal of this work was to establish methods and demonstrate the feasibility of obtaining viable primary human type I-like alveolar epithelial cells (AECs) from remnant tissue, even after(More)
Mechanical ventilation with high tidal volumes has been associated with pulmonary alveolar flooding. Understanding the mechanisms underlying cyclic stretch-induced increases in alveolar epithelial permeability may be important in designing preventive measures for acute lung injury. In this work, we assessed whether cyclic stretch leads to the generation of(More)
We found that stretching Type I rat alveolar epithelial cell (RAEC) monolayers at magnitudes that correspond to high tidal-volume mechanical ventilation results in the production of reactive oxygen species, including nitric oxide and superoxide. Scavenging superoxide with Tiron eliminated the stretch-induced increase in cell monolayer permeability, and(More)
The mechanisms underIying cyclic-stretch induced increase in pulmonary alveolar epithelial permeability are not yet fully elucidated. Here we demonstrate high levels of superoxide and nitric oxide in type 1 alveolar epithelial cells that were stretched at 12 and 37% change in surface area for different time durations. Cyclic stretch induced increase in(More)
Rat precision-cut lung slices (PCLS) were characterized as a novel ex vivo model for lung injury studies. PCLS of 250 μm in thickness were sutured onto silastic well membranes and stretched. Change in membrane surface area (%AMSA) was not significantly different from change in alveolar surface area (%AASA) throughout three cycles of stretch. At a(More)
Alveolar epithelial cells (AECs) maintain the pulmonary blood-gas barrier integrity with gasketlike intercellular tight junctions (TJ) that are anchored internally to the actin cytoskeleton. We have previously shown that AEC monolayers stretched cyclically and equibiaxially undergo rapid magnitude- and frequency-dependent actin cytoskeletal remodeling to(More)
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