Nuredin Habili

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A phytoplasma was detected in naturally diseased 'Chardonnay' grapevines exhibiting symptoms of Australian grapevine yellows disease. The use of PCR designed to amplify phytoplasma DNA resulted in detection of phytoplasma DNA in all of the diseased plants examined; no phytoplasma DNA was detected in healthy seedling grapevines. The collective restriction(More)
Complementary DNAs covering the entire RNA genome of soybean dwarf luteovirus (SDV) were cloned and sequenced. Computer analysis of the 5861 nucleotide sequence revealed five major open reading frames (ORFs) possessing conservation of sequence and organisation with known luteovirus sequences. Comparative analyses of the genome structure show that SDV shares(More)
Comparative studies of sequence motifs in the RNA polymerases and nucleic acid helicases of positive-sense RNA plant viruses have provided a new scheme for the classification of these pathogens. We propose a new luteovirus supergroup which should be added to the already described Sindbisvirus-like and picornavirus-like supergroups. Sequence motifs of(More)
Four regions covering 1247 nucleotides of the RNA genome of 20 isolates of a Vitivirus, Grapevine virus B (GVB), from three countries were analyzed. All the regions in these isolates varied in sequence as compared to the published GVB sequence. Of these, the intergenic region varied the most, with 73.2% nucleotide sequence homology, while ORF4 encoding coat(More)
Mundulla Yellows (MY) is a newly recognised lethal dieback disease of eucalypts in Australia. Descriptors of the MY syndrome have been compiled from observations of affected trees in South Australia over a 5-year period. Early, medium and late stages are defined. Symptoms resembling MY have been identified in over 70 species in all Australian states as well(More)
A total of 575 commercial grapevine (Vitis vinifera) samples from Australia have been tested for a newly emerging virus, Grapevine Pinot gris virus (Trichovirus, Betaflexiviridae) during 2015–2017. Nine samples from two states tested positive. Six of these were from New South Wales and 3 from South Australia in a total of eight varieties. All these(More)
Symptom expression of yellow speckle disease was studied in a row of 32 individual Vitis vinifera cv. Chardonnay vines in the warmer summer of 2009/2010 as compared with the cooler summer of 2011/2012 in South Australia. RT-PCR analysis showed that all these vines were positive for hop stunt viroid, grapevine yellow speckle viroid 1 (GYSVd-1) and grapevine(More)
Peach latent mosaic viroid isolates from peach and plum in Iran have been compared with an Australian isolate from nectarine. Thirteen sequence variants 336-338 nt in size were obtained. All variants clustered phylogenetically with variants reported from several hosts and countries. A total nucleic acid extract, a slightly longer than full-length RT-PCR(More)
Legume-infecting phytoplasmas identified in symptomatic tree medic (Medicago arborea), lucerne (M. sativa) and pigeonpea (Cajanus cajan) growing at a single trial site at Urrbrae, South Australia, were all shown to belong to the 16SrII phytoplasma taxonomic group, but each host species was found to be infected with a different genotype. Within one species(More)
Avocado sunblotch viroid (ASBV), coconut cadang cadang viroid (CCCV), chrysanthemum stunt viroid (CSV) and potato spindle tuber viroid (PSTV) were detected in plant extracts by dot-blot hybridization using nonradioactive photobiotin-labelled nucleic acid probes. Recombinant DNA probes, containing full-length monomer viroid inserts in the plasmid vectors(More)