Nozomi Nakanishi

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Deamination of DNA bases can create missense mutations predisposing humans to cancer and also interfere with other basic molecular genetic processes; this deamination generates deoxyinosine from deoxyadenosine. In Escherichia coli, the highly conserved endonuclease V is involved in alternative excision repair that removes deoxyinosine from DNA. However, its(More)
Cyclic AMP (cAMP) is well known to enhance tyrosine hydroxylase activity in PC12 cells. We were able to demonstrate, however, that the cellular dopamine level in PC12 was lowered by dibutyryl cAMP. Furthermore, the decrease in the cellular level of dopamine was accompanied by about a 10-fold increase in the medium. The aim of this work was to elucidate the(More)
Soluble extracts from nerve growth factor (NGF)-stimulated PC12 cells prepared by alkaline lysis show a two- to 10-fold greater ability to phosphorylate the 40S ribosomal protein S6 than do extracts from control cells. The alkaline lysis method yields a preparation of much higher specific activity than does sonication. Half-maximal incorporation of 32P from(More)
This study aimed at providing new insight into the dietary habits of the endangered Iriomote cat (Prionailurus bengalensis iriomotensis) by examining the stomach contents. Using this method allowed for more reliable representation of the diet than the analysis of scat content as used in previous studies of this subspecies. We applied five indices: frequency(More)
Vesicular amine transport is crucial for activity of monoaminergic neurons since translocation of cytosolic amine to storage vesicles is required for both exocytotic release and reuptake of the neurotransmitter. We developed a convenient assay system for vesicular amine transport based on permeabilizing the plasma membrane of pheochromocytoma PC12 cells(More)
Extracellular ATP increased the cytosolic Ca2+ level of pheochromocytoma PC12h cells, a subclonal line of PC12. This increase in Ca2+ was predominantly due to an increased Ca2+ influx rather than to intracellular mobilization of the ion. One half of the maximal increase in Ca2+ level was observed with an ATP concentration of approximately 1 microM. ADP also(More)
Effect of alcohols (methanol, ethanol, propanol, butanol, and hexanol) on nerve growth factor (NGF)-induced neurite outgrowth from pheochromocytoma PC12h cells, a subclonal line of PC12, was studied. Although the addition of alcohol alone did not induce neurite development, both the NGF-induced increase in neurite length and the frequency of neurite-bearing(More)
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