Novella Pacini

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The in vitro transformation, under anaerobic conditions, of 3- and 7-monosulfated and unsulfated bile acids, was studied in incubates of fecal flora from three healthy subjects. Chenodeoxycholic acid 7 alpha-sulfate and ursodeoxycholic acid 7 beta-sulfate were recovered unchanged, in all cultures, at the end of the incubation time. 3-Sulfated bile acids(More)
The in vitro transformation of chenodeoxycholic (CDCA), ursodeoxycholic (UDCA), and 7-keto-lithocholic (6-keto-LCA) acid by fecal specimens from five patients with cholesterol gallstones, treated with UDCA and CDCA, and five healthy control subjects was compared. Degradation of CDCA, UDCA, and 7-keto-LCA to lithocholic acid (LCA) was generally faster in(More)
The 7 alpha-dehydroxylation of cholic and chenodeoxycholic acids by mice intestinal microorganisms was studied. When all techniques, from autopsy of the animals to final incubation, are performed in oxygen-free environment, it has been found that anaerobic O2 non tolerant microorganisms might be considered the main responsible for these transformations.
14C-Carmoisine, a sulphonated azodye, 200 mg/kg b.w. (25 muCi), was administered to rats by gavage. Separation of radioactive compounds in faeces and urine of these animals was carried out by HPLC with a UV and a radioactivity detector. In addition to unmodified carmoisine, five radioactive compounds were present. The main peak showed both the retention(More)
Fifty-one strains of the genus Bifidobacterium have been found to accumulate indole-3-lactic acid in culture broth. The isolated metabolite was identified through mass and nuclear magnetic resonance spectroscopy. All the microorganisms tested, as resting cells, have been shown to be able to convert L-tryptophan into L-indole-3-lactic acid.
Chenodeoxycholic and ursodeoxycholic acid are used widely for the treatment of gallstones. A possible drawback to their utility is their conversion to lithocholic acid, which has displayed histotoxicity and mutagenicity. The 7-oleyl esters of cheno- and ursodeoxycholic acid are not degraded by fecal bacteria and may represent safer means of treatment.
The ecosystem of the dental plaque in periodontal diseases is very complex: the study of such micro-organisms, which are mostly strict anaerobes, requires the use of specific techniques under conditions of strict anaerobiosis. The aim of the present study was to design a rapid method to evaluate the activity of antimicrobials on mixed bacterial plaque of(More)
The in vitro 7α-dehydroxylation of cholic and chenodeoxycholic acids by mixed cultures of mouse cecal microorganisms was studied. Conventional anaerobic techniques and rigorous oxygen-free anaerobic experimental conditions were compared. It was found that the total number of anaerobic oxygen-intolerant microorganisms was about 10 times higher than that of(More)