Norbert Erdmann

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The impact of hypergravity and simulated weightlessness were studied to check whether cyanobacteria perceive changes of gravity as stress. Hypergravity generated by a low-speed centrifuge increased slightly the overall activity of dehydrogenases, but the increase was the same for 90 g and 180 g. The protein pattern did not show qualitative alterations(More)
The iron deficiency-dependent regulation of isiAB transcription in Synechocystis sp. PCC 6803 was analyzed by fusion of modified isiAB promoter fragments to gfp and in vivo quantification of Gfp fluorescence. For the putative Fur box only a slight repressing impact on promoter activity could be shown. In a heteroallelic fur mutant a corresponding incomplete(More)
The cyanobacteriaMicrocystis firma andSynechococcus PCC 7942 were used to test their ability for photoproduction of glucosylglycerol, sucrose, and ammonia. For this purpose, the substantial accumulation and liberation of glucosylglycerol and sucrose during osmotic shock, and ammonia during carbon limitation, were exploited. The three substances were(More)
The response of cyanobacteria to a changing osmotic environment includes the accumulation of organic osmolytes such as glucosylglycerol. The activation of the enzymes involved in glucosylglycerol synthesis [glucosylglycerol-phosphate synthase (GGPS) and glucosylglycerol-phosphate phosphatase (GGPP)] in Synechocystis sp. strain PCC 6803 by various salts and(More)
Department of Biology, University of Rostock, 1805 Rostock, FRG The biosynthetic pathway of glucosylglycerol (GG), the osmoprotective compound of Synechocystis sp. PCC 6803, was found to proceed from ADPglucose and glycerol 3-phosphate via glucosylglycerol phosphate in a two-step reaction. Using an in vitro assay it was shown that the GG-forming enzyme(More)
We introduce a new architecture for e-textbooks which contains two navigational aids: an index and a concept map. We report results from an evaluation in a university setting with 99 students. The interaction sequences of the users were captured during the user study. We found several clusters of user interaction types in our data. Three separate user types(More)
Glucosylglycerol-phosphate synthase (GGPS), the key enzyme of the glucosylglycerol biosynthesis in salt-stressed cells of Synechocystis, was biochemically analyzed in crude extracts, after partial purification by FPLC and after overexpression of the gene ggpS in Escherichia coli and purification to homogenity of the recombinant protein, respectively. These(More)
3H-serine+14C-indole were administered to tips of sterily-grown pea seedlings and of non-sterile oat coleoptiles. The Try and IAA produced were extracted and purified by paper chromatography, and their 3H/14C ratios were determined. The 3H/14C ratio of IAA was lower than the 3H/14C ratio of Try. However, the same decrease of the IAA 3H/14C ratio was found(More)
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