Nola M Erhardt

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We previously reported that pituitary adenylate cyclase-activating polypeptide (PACAP) increased cAMP in neuroblast-enriched cultures from embryonic day 3.5 chick brain. Also, the neuroblasts expressed the mRNA, peptide, and receptor for PACAP. Here, we investigated downstream effects of increased cAMP by examining PACAP's role in regulating cell numbers(More)
To investigate the involvement of pituitary adenylate cyclase- activating polypeptide (PACAP) and GH-releasing factor (GRF) during early chick brain development, we established neuroblast- enriched primary cell cultures derived from embryonic day 3.5 chick brain. We measured increases in cAMP generated by several species-specific forms of the peptides.(More)
We showed previously that early chick neuroblasts stop proliferating and undergo apoptosis when deprived of endogenous pituitary adenylate cyclase-activating polypeptide (PACAP). To identify proteins involved in these processes, we blocked the primary PACAP receptor and determined protein changes using isotope-coded affinity tag (ICAT) analysis. Cell cycle(More)
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