Nobuyuki Tanahashi

Learn More
Delayed neuronal death in the hippocampal CA1 region after transient forebrain ischemia may share its underlying mechanism with neurodegeneration and other modes of neuronal death. The precise mechanism, however, remains unknown. In the postischemic hippocampus, conjugated ubiquitin accumulates and free ubiquitin is depleted, suggesting impaired proteasome(More)
The 26 S proteasome is an evolutionarily conserved ATP-dependent protease complex that degrades poly-ubiquitinated proteins and plays essential roles in a critical part of cellular regulation. In vertebrates, the roles of the proteasome have been widely studied by use of specific inhibitors, but not genetically. Here, we generated a cell line Z(-/-/-)/Z-HA,(More)
The B protein, a subunit of lactose synthetase (EC 2.4.1. c), was crystallized from bovine skim milk and bovine mammary tissue. The B protein was identified as cu-lactalbumin, based on the following criteria: substitution in the enxymic rate assays, spectra, immunological titrations, amino acid composition, mobility on starch gel electrophoresis, molecular(More)
Prostaglandin D(2) (PGD(2)), a major cyclooxygenase product in a variety of tissues and cells, readily undergoes dehydration to yield electrophilic PGs, such as 15-deoxy-Delta(12,14)-PGJ(2) (15d-PGJ(2)). We have previously shown that 15d-PGJ(2) potently induces apoptosis of SH-SY5Y human neuroblastoma cells via accumulation of the tumor suppressor gene(More)
Manual acupuncture at Yanglingquan (GB34) is reported to have a beneficial effect on chronic liver damage. We, therefore, studied the effect of manual acupuncture at GB34 on acute liver damage. Rats were administered carbon tetrachloride (CCl(4)) or olive oil, and direct manual acupuncture was subsequently performed at GB34 or at a sham point (a(More)
The 20S proteasome is an intriguingly large complex that acts as a proteolytic catalytic machine. Accumulating evidence indicates the existence of multiple factors capable of regulating the proteasome function. They are classified into two different categories, one type of regulator is PA700 or PA28 that is reversibly associated with the 20S proteasome to(More)
Depression is a kind of mood disorder. The incidence of depressed patients has demonstrated an upward trend in recent years. Symptoms may improve with treatments such as pharmacotherapy and cognitive-behavioral therapy, but such approaches may exert strong side effects, and therapeutic effects can be slow. We studied how acupuncture stimulation would affect(More)
Acute exposure to gaseous 1,2-dibromo-3-chloropropane (DBCP) caused sex-linked recessive lethal mutations in Drosophila melanogaster males. Brood pattern analysis showed that spermatids and spermatocytes were more sensitive to the lethal-mutation induction by DBCP than were spermatogonia. The mutation induction was not observed in the stage of spermatozoa.