Nobuo Nakanishi

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Cyclic AMP (cAMP) is well known to enhance tyrosine hydroxylase activity in PC12 cells. We were able to demonstrate, however, that the cellular dopamine level in PC12 was lowered by dibutyryl cAMP. Furthermore, the decrease in the cellular level of dopamine was accompanied by about a 10-fold increase in the medium. The aim of this work was to elucidate the(More)
Although the gene encoding optineurin (OPTN) is a causative gene for glaucoma and amyotrophic lateral sclerosis, it is ubiquitously expressed in all body tissues, including the retina. To study the function of OPTN in retinal ganglion cells as well as the whole retina, we previously isolated OPTN-interacting proteins and identified the gene encoding the(More)
The concentration of fecal secretory immunoglobulin A (sIgA) in neonate and weaning piglets was measured daily from 1 day after birth to 50 days of age. The concentration of fecal sIgA started from the level of 10(4) microg/g wet feces 1 day after birth and then increased to a maximal value of up to 10(5) microg/g within a few days of birth. The values(More)
Soluble extracts from nerve growth factor (NGF)-stimulated PC12 cells prepared by alkaline lysis show a two- to 10-fold greater ability to phosphorylate the 40S ribosomal protein S6 than do extracts from control cells. The alkaline lysis method yields a preparation of much higher specific activity than does sonication. Half-maximal incorporation of 32P from(More)
BACKGROUND Mouse kallikrein 1b26 (klk1b26) protein is more abundant in male submandibular glands (SMGs) than in female ones. This sexual dimorphism has been thought to be due to increased mRNA synthesis stimulated by androgen. However, the klk1b26 protein level in female SMG is far less than that expected from the mRNA level, suggesting an additional(More)
Vesicular amine transport is crucial for activity of monoaminergic neurons since translocation of cytosolic amine to storage vesicles is required for both exocytotic release and reuptake of the neurotransmitter. We developed a convenient assay system for vesicular amine transport based on permeabilizing the plasma membrane of pheochromocytoma PC12 cells(More)
Extracellular ATP increased the cytosolic Ca2+ level of pheochromocytoma PC12h cells, a subclonal line of PC12. This increase in Ca2+ was predominantly due to an increased Ca2+ influx rather than to intracellular mobilization of the ion. One half of the maximal increase in Ca2+ level was observed with an ATP concentration of approximately 1 microM. ADP also(More)
Effect of alcohols (methanol, ethanol, propanol, butanol, and hexanol) on nerve growth factor (NGF)-induced neurite outgrowth from pheochromocytoma PC12h cells, a subclonal line of PC12, was studied. Although the addition of alcohol alone did not induce neurite development, both the NGF-induced increase in neurite length and the frequency of neurite-bearing(More)
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