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This paper describes a new series of staining methods which can discriminatively demonstrate every structure of the nervous system, including axons and capillaries, in animal and human materials. Methods described in this paper consist of one primary stain, luxol fast blue-periodic acid Schiff-hematoxylin (LPH) and six different subsidiary staining methods.(More)
A partial nucleotide sequence of the mRNA encoding a major part of elongation factor 1 alpha (EF1 alpha) from a mitochondria-lacking protozoan, Giardia lamblia, was reported, and the phylogenetic relationship among lower eukaryotes was inferred by the maximum-likelihood and maximum-parsimony methods of protein phylogeny. Both the methods consistently(More)
Chronologic and morphometric changes in the inferior olivary nucleus of the human medulla oblongata were studied in eight cases of primary pontine hemorrhage with different survival periods. To measure the olivary areas and analyze the neuronal and glial components, an optic electronic planimeter was used. A desk-top computer was also used for the(More)
The development of the human cochlear nucleus was studied in serial sections of the brain of 12 fetuses at 12-40 weeks of gestation, an infant at 2 months of age and an adult of 63 years using an electronic planimeter with a computer. Morphometric analysis of the development of the ventral cochlear nucleus showed that its development accelerates after 18(More)
Anatomical development of the human mesencephalic trigeminal nucleus was assessed quantitatively and qualitatively, using serial sections of brains, with the help of a computer-assisted image-analyser. Five fetuses at 16, 18, 21, 23 and 27 weeks of gestation; five neonates at 30, 32, 33, 35 and 40 weeks of gestation; a two-month-old infant and a 63-year-old(More)
The morphometric features of the motor trigeminal nucleus of the developing human brain were examined using complete serial celloidin sections of 13 brains, including 10 fetal brains. Our results suggest that the developmental processes of the motor trigeminal nucleus should be divided into four stages as follows: (1) primary stage, characterized by the(More)
Ghrelin is a novel peptide that stimulates the release of growth hormone from the pituitary and is involved in hypothalamic feeding regulation. A pre-embedding immunostaining technique was used to study the ultrastructure and synaptic relationships of ghrelin-containing neurons in the rat arcuate nucleus (ARC). Ghrelin-like immunoreactive (ghrelin-LI)(More)
The morphometric development of the human cerebellar nuclei was examined in 9 fetuses (16-40 weeks of gestation; WG), an infant (2 months old) and 2 adults (16 and 63 years old). With the morphological observation of serial sections of the brain containing the cerebellar nuclei, the authors measured sections to get several morphometric parameters: the(More)
We examined numbers and sizes of anterior horn cells (AHCs) of the human spinal cord at C6 level with aging process. Spinal cords were obtained from twenty-four male cadavers, age ranged from 41 to 97 years without any accompanying pathological changes of the spinal cord. For quantitative investigation of the AHCs, spinal cord segments were embedded in(More)
Nerve fiber analyses were performed on the human vestibulocochlear nerve stained with Luxol fast blue-periodic acid-Schiff-hematoxylin with use of a combination of an image-analyzer and a computer. The axons were counted and their transverse (cross-sectional) areas were measured in 12 individuals. The average numbers of axons in each vestibular and cochlear(More)