Nistor Cristina

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A Toxoplasma gondii genomic library was constructed in lambda EMBL3. Repeated fragments were detected by hybridization with radiolabeled total DNA from the parasite and one recombinant was chosen due to its strong hybridization signal. By using electrophoretic and hybridization analysis, four cross-hybridizating restriction fragments were selected and(More)
A repetitive original DNA sequence, TGR1E, was cloned and sequenced, then used to develop a polymerase chain reaction (PCR) test for detecting Toxoplasma gondii. Preliminary studies were performed using purified T. gondii DNA or a lysate of purified T. gondii cells [7], with or without a leukocyte lysate. A negative correlation was evidenced between(More)
In recent years, toxoplasmosis has become one of the most frequent and life-threatening opportunistic infections in AIDS patients. Despite strict clinical follow-up and repeated biological examinations, its diagnosis remains difficult to establish in the context of immunodeficiency because of the poor predictive value of serology. The aim of the study was(More)
Toxoplasmosis is one of the latest parasitic diseases to have benefited from molecular biological study (1989). To our knowledge we were one of the first to have cloned several DNA probes using genomic DNA from Toxoplasma gondii. One of these probes, TGR1E, has been sequenced and has enabled the selection and chemical synthesis of 4 oligonucleotides (22 to(More)
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