Nisha T. Govender

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A highly efficient and reproducible Agrobacterium-mediated transformation protocol for Ganoderma boninense was developed to facilitate observation of the early stage infection of basal stem rot (BSR). The method was proven amenable to different explants (basidiospore, protoplast, and mycelium) of G. boninense. The transformation efficiency was highest (62%)(More)
Shoot and inflorescence are central physiological and developmental tissues of plants. Flowering is one of the most important agronomic traits for improvement of crop yield. To analyze the vegetative to reproductive tissue transition in Jatropha curcas, gene expression profiles were generated from shoot and inflorescence tissues. RNA isolated from both(More)
Basal stem rot, caused by the basidiomycete fungus, Ganoderma boninense, is an economically devastating disease in Malaysia. Our study investigated the changes in lignin content and composition along with activity and expression of the phenylpropanoid pathway enzymes and genes in oil palm root tissues during G. boninense infection. We sampled control(More)
A three month study to evaluate costs associated with the utilisation of antimicrobial drugs was undertaken in the Intensive Care Unit of the Johannesburg hospital. A total of 62 patients were entered in the study. Coagulase-negative Staphylococcus was found to be the most prominent bacterial pathogen (53%) followed by Acinetobucter bauma?mii (30.6%).(More)
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