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Systems-level understanding of how Propionibacterium acidipropionici respond to propionic acid stress at the microenvironment levels: mechanism and application.
TLDR
It was indicated that the acid tolerance of P. acidipropionici was systematically regulated, and the increased arginine, aspartic acid, and glutamic acid concentrations helped to resist the acidic environment by consuming more H⁺ and generating more ATP and NH₃. Expand
Microbial response to acid stress: mechanisms and applications
TLDR
This review summarizes acid stress response mechanisms of microbial cells, illustrates the application of microbial acid tolerance in industry, and prospect the introduction of systems and synthetic biology to further explore the acid tolerance mechanisms and construct a microbial cell factory for valuable chemicals. Expand
Pathway engineering of Propionibacterium jensenii for improved production of propionic acid
TLDR
Propionic acid production in P. jensenii with ppc overexpression as well as ldh deletion was investigated, which resulted in further increases in PA titer to 34.93 ± 2.99 g·L−1 in a fed-batch culture. Expand
Comparative metabolomics analysis of the key metabolic nodes in propionic acid synthesis in Propionibacterium acidipropionici
TLDR
Results indicated that the amounts of metabolic intermediates of glycolysis, the Wood–Werkman cycle, and amino acid metabolism differed markedly between parental P. acidipropionici and its mutants, and exogenous addition of key metabolites (precursors and amino acids) was performed to improve PA production. Expand
A versatile genetic control system in mammalian cells and mice responsive to clinically licensed sodium ferulate
TLDR
An FA/SF-adjustable transcriptional switch controlled by the clinically licensed drug SF is described and it is demonstrated that SF-responsive switches can be engineered to control CRISPR-Cas9 systems for on-command genome/epigenome engineering. Expand
Microbial response to environmental stresses: from fundamental mechanisms to practical applications
TLDR
The modus operandi by which stresses act on cellular components, as well as the corresponding resistance mechanisms developed by microorganisms, are summarized and the applications of these stress resistance mechanisms on the production of industrially important chemicals are discussed. Expand
Understanding of how Propionibacterium acidipropionici respond to propionic acid stress at the level of proteomics
TLDR
A comparative proteomics study of P. acidipropionici and secretory protein glyceraldehyde-3-phosphate dehydrogenase and ATP synthase subunit α in Escherichia coli BL21 improved PA and acetic acid tolerance; overexpression of NADH dehydrogenases and methylmalonyl-CoA epimerase improved PA tolerance. Expand
Metabolic engineering of acid resistance elements to improve acid resistance and propionic acid production of Propionibacterium jensenii
TLDR
This study provides an effective strategy for improving PA production in propionibacteria by engineering elements of the arginine deaminase and glutamate decarboxylase systems that were overexpressed in P. jensenii. Expand
Engineering a far-red light-activated split-Cas9 system for remote-controlled genome editing of internal organs and tumors.
TLDR
A far-red light-activated split-Cas9 system that can robustly induce gene editing in both mammalian cells and mice is described and FAST was demonstrated to achieve FRL-triggered editing of the PLK1 oncogene in a mouse xenograft tumor model. Expand
Comparative genomics and transcriptomics analysis‐guided metabolic engineering of Propionibacterium acidipropionici for improved propionic acid production
TLDR
A metabolic engineering strategy to regulate the synthesis of lactic acid and acetic acid that will reduce by‐products significantly and increase the PA yield by 12.2% to 10.31 ± 0.84 g/g DCW is proposed. Expand
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