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Structurally isomeric complexes formed between homopyrimidine bis-PNAs (T(2)JT(2)JT(4)-linker-T(4)CT(2)CT(2)) and single- and double-stranded DNA targets were investigated. These complexes are triplexes designated S1, S2 and S3 in order of increased mobility by polyacrylamide gel electrophoresis. It is shown that the S3 isomer is formed only on(More)
Dissociation kinetics of triplexes formed by molecules of peptide nucleic acid (PNA) and DNA have been studied. The complexes consisted of oligomeric PNA containing 10 thymine bases and the dA(10) target incorporated in single-stranded (ssDNA) or double-stranded DNA (dsDNA). Their dissociation was followed by means of the gel mobility shift assay at various(More)
The distribution of guanine-cytosine (GC) pairs in the DNA of the highly oncogenic simian adenovirus type 7 (SA7) and the non-oncogenic human adenovirus type 6 (Ad6) has been studied by thermal denaturation and CsC1 density-gradient centrifugation. The differential of the DNA thermal denaturation curves shows the presence of pronounced peaks which indicates(More)
We studied modification of double-stranded DNA with chemically active homologous oligonucleotide derivatives carrying an alkylating 4-[N-2-chloroethyl-N-methylamino)benzyl]aminogroup in DNA recombination reaction promoted by E. coli RecA protein. It was shown that this chemical group does not prevent the formation of a complex between RecA protein and(More)
343 Despite the long standing investigations of the linker histones in many laboratories worldwide, their biological role and many physicochemical aspects of their interactions with DNA remain unclear. Tradi tionally, histone H1 and its variants were mainly con sidered as the agents that provide a tight compaction of chromatin and the related regulation of(More)
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