Nina Lemo

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To investigate the degree of genetic variability of bovine leukemia virus (BLV) strains circulating in Croatia, 29 isolates from the six largest dairy farms were examined by PCR for a segment of the gp51 env gene, followed by DNA sequencing and phylogenetic analysis. The nucleotide sequences were compared with other previously characterized BLV strains from(More)
Bovine viral diarrhoea virus (BVDV) causes a disease that has a wide range of clinical symptoms in domestic and wild ruminants. It is a major problem in cattle and causes significant economic losses in the cattle industry. The virus infects bovines of all ages and causes both immunosuppression and reproductive, respiratory and digestive disorders. Cattle(More)
Between 2007 and 2011, a total of 1937 sera samples and fi ve spleen samples from nine Croatian dairy Between 2007 and 2011, a total of 1937 sera samples and fi ve spleen samples from nine Croatian dairy herds were tested for the bovine viral diarrhea virus (BVDV) using virus isolation and the immunoperoxidase herds were tested for the bovine viral diarrhea(More)
The virus neutralisation test is used for the quantitation of specific antibodies in serum samples. However, the success of the test depends on the quality of samples. In the case of poor quality samples, a cytotoxic effect can be observed and the results of the test can be compromised. Additionally, the cytotoxic effect limits the use of different(More)
In this report a persistently infected cow was under surveillance for three years. In that period the level of antibodies and level of viremia were determined. Persistent infection was confi rmed in blood serum samples taken at 3 week intervals and tested on the virus antigen by antigen enzyme-linked immunosorbent assay and by inoculation in cell culture,(More)
To eliminate cytotoxic effects of colostrum on cells, a modified virus neutralization test (VNT) for the detection of Bovine viral diarrhea virus-specific neutralizing antibodies in colostrum was developed. The new test was compared to the World Organization for Animal Health-recommended VNT and the results evaluated. The agreement of the new test compared(More)
Sylvatic rabies has been present in Croatia for more than three decades, with the red fox (Vulpes vulpes) as the main reservoir. The present epidemic of sylvatic rabies in Croatia started already in 1977 and in the past ten years the disease has become enzootic in the entire country and thus represents a considerable veterinary and public health threat. A(More)
BACKGROUND Bovine viral diarrhea is a contagious disease of domestic and wild ruminants and one of the most economically important diseases in cattle. Bovine viral diarrhea virus belongs to the genus Pestivirus, within the family Flaviviridae. The identification and elimination of the persistently infected animals from herds is the initial step in the(More)
The applicability and reliability were investigated of antigen enzyme linked immunosorbent assay (Ag The applicability and reliability were investigated of antigen enzyme linked immunosorbent assay (Ag ELISA) and reverse transcription polymerase chain reaction (RT-PCR) for the diagnosis of bovine viral ELISA) and reverse transcription polymerase chain(More)
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