Nilesh Chandra Sharma

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Growth of Sesbania seedlings in chloroaurate solution resulted in the accumulation of gold with the formation of stable gold nanoparticles in plant tissues. Transmission electron microscopy revealed the intracellular distribution of monodisperse nanospheres, possibly due to reduction of the metal ions by secondary metabolites present in cells. X-ray(More)
Deterioration in water quality caused by the movement of excessive soil P has created a condition necessary for the development of a sustainable P remediation technology. In this investigation, the phytoremediation potential of Gulf and Marshall ryegrass (Lolium multiflorum) grown in a greenhouse was determined under varying conditions of soil P(More)
Effects of lead (Pb) and chelators, such as EDTA, HEDTA, DTPA, NTA and citric acid, were studied to evaluate the growth potential of Sesbania drummondii in soils contaminated with high concentrations of Pb. S. drummondii seedlings were grown in soil containing 7.5 g Pb(NO(3))(2) and 0-10 mmol chelators/kg soil for a period of 2 and 4 weeks and assessed for(More)
Seedlings of Sesbania drummondii were grown in 500 mg l-1 Pb(NO3)2 in presence and absence of chelators: EDTA, DTPA and HEDTA for 4 weeks. Plants were assayed for activities of the antioxidant enzymes: ascorbate peroxidase (APX), guaiacol peroxidase (GPX), catalase (CAT), superoxide dismutase (SOD) and glutathione (gamma-glutamyl-cysteinyl-glycine) content.(More)
In the search for a suitable plant to be used in P phytoremediation, several species belonging to legume, vegetable and herb crops were grown in P-enriched soils, and screened for P accumulation potentials. A large variation in P concentrations of different plant species was observed. Some vegetable species such as cucumber (Cucumis sativus) and yellow(More)
A protocol is described for rapid and large-scale in vitro clonal propagation of the valuable medicinal herb Centella asiatica (L.) by enhanced axillary bud proliferation in nodal segments isolated from mature plants. Although bud break was dependent on BA supply, the synergistic combination of 22.2 μM BA and 2.68 μM NAA induced the optimum frequency (91%)(More)
Search for plant species - prodigious in P use - is important for both P-sufficient and -deficient conditions. Gulf and Marshall ryegrass (Lolium multiflorum), grown in sterile media containing different organic P substrates (AMP, ATP, GMP, and IHP), exhibited high rates of growth and shoot P concentrations. Growth increase in Gulf was significantly greater(More)
Lead phytoextraction can be economically feasible only when the developed systems employ high biomass plants that can accumulate greater than 1% Pb in their shoots. In this study Sesbania drummondii, a leguminous shrub occurring in the wild, was used to demonstrate its capability for greater than 1% Pb accumulation in shoots when grown in a Pb-contaminated(More)
Agricultural and animal husbandry practices combined with soil composition have caused phosphate overloading of farmlands in different parts of the U.S. and Europe. Movement of soluble phosphates (Pi) from phosphorus enriched soils results in degradation of natural aquatic systems, triggering serious environmental problems. Remediation of such sites using(More)
Plumbagin (1) is a naphthoquinone constituent of plants that have been used in traditional systems of medicine since ancient times. In the present study, the role of 1 was examined on the amelioration of ulcerative colitis, an inflammatory bowel disease that is not curable currently. Plumbagin was tested at a dose of 6-10 mg/kg body weight in acute and(More)