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Plant Glutathione Transferases in Abiotic Stress Response and Herbicide Resistance
This chapter discusses the involvement of plant GSTs in abiotic stress response with focus on metabolism-based herbicide resistance and attempts to give an overview of their catalytic roles and in planta function. Expand
Structure, Evolution and Functional Roles of Plant Glutathione Transferases
This chapter focuses on plant GSTs and attempts to give an overview of their evolution, catalytic function and structural biology. Expand
Concluding the trilogy: The interaction of 2,2′‐dihydroxy‐benzophenones and their carbonyl N‐analogues with human glutathione transferase M1‐1 face to face with the P1‐1 and A1‐1 isoenzymes involved
The outcome of the isoenzyme trilogy identified good binder leads for the investigated GSTs involved in MDR, and 5‐ or 5‐Bromo‐ or phenyl‐substituted inhibitors, having a H‐bonded oxime weakly acidic group of a small volume, are optimal candidates for binding hGSTM1‐1. Expand
Glutathione analogues as substrates or inhibitors that discriminate between allozymes of the MDR‐involved human glutathione transferase P1‐1
It is anticipated that when GSH analogues with selective inhibitory or catalytic binding, were conjugated to allozyme‐selective inhibitors of hGSTP1‐1, the derived leads would be useful for the designing of novel chimeric inhibitors against the MDR‐involved hG STP 1‐1 allozymes. Expand
Comparative structural and functional analysis of phi class glutathione transferases involved in multiple-herbicide resistance of grass weeds and crops.
Kinetic analysis of substrate specificity using a range of thiol substrates and xenobiotic compounds suggested that all enzymes display a broad range of specificity and are capable of detoxifying major stress-induced toxic products. Expand
Ligand Fishing: An Approach for the Discovery of Inhibitors from Complex Biological Mixtures.
This chapter provides protocols for the synthesis of affinity adsorbent and its application in off-line ligand-fishing procedure for a 6His-tagged glutathione transferase (GST). Expand
Sol–gel immobilization of haloalkane dehalogenase from Bradyrhizobium japonicum for the remediation 1,2-dibromoethane
Abstract Haloalkane dehalogenases catalyze the hydrolytic cleavage of carbon–halogen bonds in a broad range of environmental pollutants such as aliphatic mono-, di-, and polyhalogenated alkanes. FromExpand
Determination of Half-Maximal Inhibitory Concentration of an Enzyme Inhibitor.
This chapter describes an experimental procedure for the determination of the IC50 value of an enzyme inhibitor, and the relationship between IC50 and Ki is also discussed. Expand
Peptide cell-display for selection of inhibitors against human glutathione transferase P1-1 (hGSTP1-1) allozymes
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Combinatorial design, selection and synthesis of peptide inhibitors against human glutathione transferase p1-1
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