Nikolai N. Kolesnikov

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The Xist locus plays a central role in the regulation of X chromosome inactivation in mammals, although its exact mode of action remains to be elucidated. Evolutionary studies are important in identifying conserved genomic regions and defining their possible function. Here we report cloning, sequence analysis, and detailed characterization of the Xist gene(More)
Two genomic clones, pC1.2 and p20D (containing inserts of 2.0 and 1.6 kb, respectively) were isolated from the A2b region to polytene chromosome IV of Chironomus thummi thummi salivary gland cells. Upon in situ hybridization to polytene chromosomes of C. thummi thummi and C. thummi piger, p20D DNA hybridized mainly over the A2b region of chromosome IV,(More)
A 2.3-kb genomic clone has been isolated from the region where the tissue-specific puff, Balbiani ring a (BRa), is found on chromosome IV of the special lobe of Chironomus thummi salivary gland cells. The clone was characterized by nucleotide sequence analysis. Two clusters of direct tandem repeats were identified, as well as large and small open reading(More)
The Xist gene belongs to the class of long noncoding regulatory RNA genes which play a key role in the process of inactivation of one of the X chromosomes in females of placental mammals. Based on inter-specific comparative sequence analysis performed using a set of bioinformatic programs and approaches, the exon-intron gene structure was first described in(More)
Synthesis and glycosylation of larval salivary gland secretory proteins of Chironomus thummi were analyzed with respect to cell specific differences in the Balbiani ring (BR) pattern and glycoprotein composition of secretion formerly detected by histochemical staining procedures. In the secretion of a special cell type in salivary glands, which is(More)
AIMS In this study, we determined the expression level of miRNAs and the induction of CYP1A1 and CYP2B1 in the livers and ovaries of female Wistar rats treated with DDT, benzo[a]pyrene (BP), and 3-methylcholanthrene (MC). This study compared CYP1A/2B induction and miRNA expression levels to cast light on a possible role of miRNA in the tissue-specific(More)
The processes of involved in the accumulation and secretion of adhesive mucoprotein (“glue”) in third instar larvae ofD. melanogaster are described. The RNA templates for glue protein seem to be synthesized within 72–85 hours after oviposition, because treatment of larvae at this age with actinomycin D inhibits the accumulation of the secretion. The(More)
A 1.7 kb long transposable element called TECth1 was found in the 3′ flanking region of aChironomus thummi Balbiani ring gene. As shown by sequence comparison with a second copy, TECthl is characterized by a perfect terminal inverted repeat of 17 by flanked by a duplicated target site of 8 bp, four internal imperfect inverted repeats of 17 to 26 by and(More)
Methods of obtaining synchronously developing larvae were comparatively evaluated. Larvae were selected by distinct morphological features: time of hatching, second larval moult, pupation. Both virgin and non-virgin females were taken in the experiment. The degree of synchronism was estimated by the interval during which pupation took place in all larvae of(More)
After the radiation of primates and rodents, the evolution of X-chromosome inactivation centers in human and mouse (XIC/Xic) followed two different directions. Human XIC followed the pathway towards transposon accumulation (the repeat proportion in the center constitutes 72%), especially LINEs, which prevail in the center. On the contrary, mouse Xic(More)