Nikola Krasnov

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The paper deals with investigation of silver ion interaction with sulfur-bearing amino acids and cysteine-bearing peptides using an electrospray ionisation orthogonal ion introduction time-of-flight (ESI-o-TOF) mass spectrometer. It has been shown that Cys and Hcy demonstrate the largest affinity for silver, both in relation to methionine and cysteine(More)
A reaction of high-temperature solid-phase catalytic isotope exchange (HSCIE) was studied for the preparation of tritium- and deuterium-labeled ligands of glutamate and dopamine receptors. Tritium-labeled (5S,10R)-(+)-5-methyl-10,11-dihydro-5H-dibenzo[a,d]cyclopenten-5,1-imine ([G-3H]MK-801) and R(+)-7-hydroxy-N,N-di-n-propyl-2-aminotetraline(More)
RATIONALE Although the electrospray method is widely used in biomedical mass spectrometry and ion-drift spectrometry, the electrospray system can generate large drops that are the cause of electrical breakdowns, and of the fast clogging of the inlet diaphragm of the differential pumping first stage and system of ion transportation to the analyzer. Thus, a(More)
N-Arachidonoyl (AA) derivatives of amino acids (glycine, phenylalanine, proline, valine, γ-aminobutyric acid (GABA), dihydroxyphenylalanine, tyrosine, tryptophan, and alanine) and peptides (Semax, MEHFPGP, and PGP) were synthesized in order to study the biological properties of acylamino acids. The mass spectra of all the compounds at atmospheric pressure(More)
The possibility of interaction of 0.1 mg/mL acetylsalicylic acid with purified human and rat globin in vitro during 24 h at 37 degrees C was investigated. The rat globin can be modified with acetylsalicylic acid on aminoacid residues K-17, K-57, K-91, K-140 in alpha subunit as well as on K-18, K-77 in beta subunit. The human globin can be modified with(More)
The possibility of interaction of 0.1 mg/mL acetylsalicylic acid (ASA) with purified human and rat globin proteins for 24 h in vitro was investigated. It was shown that following the interaction with ASA rat globin is modified at Lys17, Lys57, Lys91, and Lys140 amino acid residues of the α-subunit as well as at Lys18 and Lys77 of the β-subunit, whereas(More)
An experimental high-resolution ion-drift spectrometer is described. Operating characteristics were improved by installation of a second grid gate in the center of the drift space. The ion-drift analyzer setups and the ranges of amplitude, length and temporary delay of control impulses were identified in the course of testing. The article gives illustration(More)
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