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Reconstitution of long and short patch mismatch repair reactions using Saccharomyces cerevisiae proteins
Significance This study demonstrates mismatch repair (MMR) reactions reconstituted in vitro with purified Saccharomyces cerevisiae proteins. Biochemical analysis of MMR in vitro showed that MMRExpand
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Dominant Mutations in S. cerevisiae PMS1 Identify the Mlh1-Pms1 Endonuclease Active Site and an Exonuclease 1-Independent Mismatch Repair Pathway
Lynch syndrome (hereditary nonpolypsis colorectal cancer or HNPCC) is a common cancer predisposition syndrome. Predisposition to cancer in this syndrome results from increased accumulation ofExpand
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Mlh2 Is an Accessory Factor for DNA Mismatch Repair in Saccharomyces cerevisiae
In Saccharomyces cerevisiae, the essential mismatch repair (MMR) endonuclease Mlh1-Pms1 forms foci promoted by Msh2-Msh6 or Msh2-Msh3 in response to mispaired bases. Here we analyzed the Mlh1-Mlh2Expand
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Activation of Saccharomyces cerevisiae Mlh1-Pms1 Endonuclease in a Reconstituted Mismatch Repair System*
Background: Biochemical analysis of S. cerevisiae MMR mutants has been limited by a lack of reconstituted MMR reactions. Results: 3′ nick-directed Mlh1-Pms1-dependent endonuclease and reconstitutedExpand
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Mispair-specific Recruitment of the Mlh1-Pms1 Complex Identifies Repair Substrates of the Saccharomyces cerevisiae Msh2-Msh3 Complex*
Background: Msh2-Msh6 and Msh2-Msh3 bind to and initiate the repair of mismatched DNA. Results: Purified Msh2-Msh3 binds specific base:base mispairs and small to large insertion/deletions andExpand
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Reconstitution of Saccharomyces cerevisiae DNA polymerase ε-dependent mismatch repair with purified proteins
Significance By performing reconstitution studies with purified Saccharomyces cerevisiae proteins, this study provides the first demonstration of eukaryotic mismatch repair (MMR) reactions dependentExpand
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