Nikhil V Mittal

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Cells of Escherichia coli under conditions of certain cellular stresses excrete attractants. Cells of chemotactic strains respond to these excreted signaling molecules by moving up their local concentration gradients and forming different types of stable multicellular structures. Multicellular clusters are the simplest among these structures. Fluorescence(More)
We present a novel technique to accurately position single cells on a substrate using negative dielectrophoresis and cell-substrate adhesion. The cells are suspended in physiological media throughout the patterning process. We also verify the biocompatibility of this method by demonstrating that the patterned cells proliferate and show normal morphology. We(More)
The MIT Faculty has made this article openly available. Please share how this access benefits you. Your story matters. Abstract An improved understanding of the role of extracellular factors in controlling the embryonic stem cell (ESC) phenotype will aid the development of cell-based therapies. While the role of extracellular factors in controlling the(More)
A common assumption made when performing in vitro cellular assays is that the concentration of substances in the culture system is uniform. However, since the cells that internalize and secrete substances reside at the bottom of the well, it is conceivable that a concentration gradient could arise across the fluid layer. Importantly, the concentration of a(More)
Six hypothetical proteins of Halobacterium salinarum NRC-1 VNG0546c, VNG2021c, VNG2549c, VNG0683c, VNG2351c and VNG1475c were studied. Expasy's ProtParam study of physico-chemical properties showed that these halophilic proteins are acidic in character and stable in nature having high extinction coefficient ranging from 26164.9 to 57758.3 M 1 cm–1, high(More)
This research involves the development of numerous microfab-ricated sorting cytometer architectures for genetic screening of complex phenotypes in biological cells. Our various approaches combine the ability to observe and isolate individual mutant cells within surveyed populations. In this work we merge benefits of both microscopy and flow-assisted cell(More)
The ability to place cells at specific locations on a substrate is a useful tool to study and engineer interactions between cells [1], perform image-based cell selection [2], and create cell-based bio-sensors [3]. The ability to pattern with single-cell resolution is necessary in order to perform studies of single-cell physiology in which these cells are(More)
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