Nicole van Uden

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Acetic acid at concentrations as may occur during vinification and other alcoholic yeast fermentations induced death of glucose-grown cell populations of Saccharomyces cerevisiae IGC 4072 at temperatures at which thermal death was not detectable. The Arrhenius plots of specific death rates with various concentrations of acetic acid (0-2%, w/v) pH 3.3 were(More)
Glucose-repressed cells of the yeast Pichia ohmeri IGC 2879 transported glucose by facilitated diffusion. Derepression led to the formation of a glucose/proton symport and the simultaneous reduction of the facilitated diffusion capacity by about 70%. Cycloheximide prevented this interconversion indicating its dependence on de novo protein synthesis(More)
Saccharomyces cerevisiae IGC4072 grown in lactic acid medium transported lactate by an accumulative electroneutral proton-lactate symport with a proton-lactate stoichiometry of 1:1. The accumulation ratio measured with propionate increased with decreasing pH from ca. 24-fold at pH 6.0 to ca. 1,400-fold at pH 3.0. The symport accepted the following(More)
The small GTPase rab4a is associated with early endocytic compartments and regulates receptor recycling from early endosomes. To understand how rab4a mediates its function, we searched for proteins which associate with this GTPase and regulate its activity in endocytic transport. Here we identified rabaptin4, a novel effector molecule of rab4a. Rabaptin4 is(More)
Metschnikowiella xobellii sp.nov. and M . krissii sp.nov. are described and Latin diagnoses given. Both species form on VS agar (Wickerham, 1951) club-shaped asci containing a single needle-shaped ascospore and are capable of parasitizing Daphnia magna under experimental conditions. M . xobellii differs from M . krissii by its capacity to ferment glucose(More)
The temperature profile of growth and thermal death of the xylose-fermenting yeast Candida shehatae was dissociative. The ARRHENIUS plot of growth lacked a descending supraoptimal branch and the specific growth rate at the maximum temperature for growth (around 31 degrees C) was not significantly different from its values at the other temperatures studied(More)
A strain of Lipomyces kononenkoae earlier proposed for industrial starch bioconversion was found to have a dissociative temperature profile. The Arrhenius plot of sustained exponential growth displayed a single branch between the optimum (32-33 degrees C) and the maximum (about 35 degrees C) temperature for growth while the extrapolated Arrhenius plots of(More)