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The Xist locus plays a central role in the regulation of X chromosome inactivation in mammals, although its exact mode of action remains to be elucidated. Evolutionary studies are important in identifying conserved genomic regions and defining their possible function. Here we report cloning, sequence analysis, and detailed characterization of the Xist gene(More)
AIMS In this study, we determined the expression level of miRNAs and the induction of CYP1A1 and CYP2B1 in the livers and ovaries of female Wistar rats treated with DDT, benzo[a]pyrene (BP), and 3-methylcholanthrene (MC). This study compared CYP1A/2B induction and miRNA expression levels to cast light on a possible role of miRNA in the tissue-specific(More)
X inactivation, the transcriptional silencing of one of the two X chromosomes in female mammals, achieves dosage compensation of X-linked genes relative to XY males. In eutherian mammals X inactivation is regulated by the X-inactive specific transcript (Xist), a cis-acting non-coding RNA that triggers silencing of the chromosome from which it is(More)
The processes of involved in the accumulation and secretion of adhesive mucoprotein ("glue") in third instar larvae ofD. melanogaster are described. The RNA templates for glue protein seem to be synthesized within 72-85 hours after oviposition, because treatment of larvae at this age with actinomycin D inhibits the accumulation of the secretion. The(More)
Methods of obtaining synchronously developing larvae were comparatively evaluated. Larvae were selected by distinct morphological features: time of hatching, second larval moult, pupation. Both virgin and non-virgin females were taken in the experiment. The degree of synchronism was estimated by the interval during which pupation took place in all larvae of(More)
After the radiation of primates and rodents, the evolution of X-chromosome inactivation centers in human and mouse (XIC/Xic) followed two different directions. Human XIC followed the pathway towards transposon accumulation (the repeat proportion in the center constitutes 72%), especially LINEs, which prevail in the center. On the contrary, mouse Xic(More)
MicroRNAs are known as a posttranscriptional negative regulators of gene expression by binding to the 3'UTP of target mRNAs in cytoplasm. More than 1600 microRNAs expressed in human cells, are involved in the regulation of embryogenesis, differentiation, cell cycle, apoptosis, senescence, thus determining cell fate. Up to 60 % of protein coding genes are(More)
The process of mucoprotein (glue) accumulation is described. This substance is secreted by salivary glands of the III instar larvae of Drosophila melanogaster. Granules of the secretory substance appear in the glandular cells 90 hrs after egg laying. Its secretion takes place within 120 hrs. The template RNA of glue proteins appears to be synthesized(More)
A 1.7 kb long transposable element called TECth1 was found in the 3' flanking region of a Chironomus thummi Balbiani ring gene. As shown by sequence comparison with a second copy, TECth1 is characterized by a perfect terminal inverted repeat of 17 bp flanked by a duplicated target site of 8 bp, four internal imperfect inverted repeats of 17 to 26 bp and(More)
Synthesis and glycosylation of larval salivary gland secretory proteins of Chironomus thummi were analyzed with respect to cell specific differences in the Balbiani ring (BR) pattern and glycoprotein composition of secretion formerly detected by histochemical staining procedures. In the secretion of a special cell type in salivary glands, which is(More)