Nicolay G. Schuppe

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Reverse transcription intermediate forms (minus and plus strong-stop DNA) are detected in Drosophila melanogaster cultured cells for mobile dispersed genetic elements mdg1, mdg3, and mdg4 (gypsy). The mdg elements studied possess a common mechanism of reverse transcription, despite their structural differences, and the comparative analysis of intermediate(More)
Extrachromosomal supercoiled circular copies of mobile dispersed genetic elements mdg1, mdg3 and copia have been isolated from 67J25D and Kc lines of cultured Drosophila melanogaster cells. With the exception of the copia element in the line Kc, all circular molecules contain only one long terminal repeat. Both whole-length circular copies and molecules(More)
Virus-like particles (VLPs) possessing reverse transcriptase activity are persistently present in Drosophila melanogaster cultured cells and are formed in yeast induced for transposition. Different retrotransposon transposition intermediates consistent with those expected from the model of reverse transcription pathway of retrotransposon transposition have(More)
DNA-RNA complexes were detected in cultured Drosophila melanogaster cells. These complexes are practically perfect hybrid molecules of mobile dispersed genes DNA (mdg1 and mdg3) and suitable poly(A)+RNA. Three species of single-stranded DNA have been found in hybrid molecules: full-sized mdg DNA containing either one or two LTRs (both minus strands) and(More)
The quantity of ribosomal RNA genes and the rate of ribosomal RNA synthesis has been studied in established cell lines of Drosophila melanogaster with diploid, polyploid and hemizygous karyotypes and in larval diploid cells carrying 1, 2, 3 or 4 nucleolar organizer regions. The rate of rRNA synthesis has been shown to depend on the number of sex chromosomes(More)
A new established cell line 79f7Dv3g, ofDrosophila virilis consisting initially of male and female cells and represented now, after 6 yr of cultivation, only by male cells is described. The population doubling time is 36 h at 25° C. The cell culture is also able to grow in serum-free media for an indefinite time without special selection and has a(More)
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