Nicolas de Saint

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The effects on the channel characteristics of four single amino acid substitutions in OmpF porin and of a deletion mutant in the constriction loop L3 have been studied. These mutations are all(More)
The sole histidine residue in OmpF porin was replaced by threonine using site-directed mutagenesis. This exchange affected neither channel properties nor channel structure, as determined by X-ray(More)
The outer-membrane (OM) permeability of the psychrotrophic bacterium Pseudomonas fluorescens strain MF0 for the beta-lactam mezlocillin is increased at the optimum growth temperature (28 degrees C)(More)
We have established a reconstitution method of the detergent-solubilized recombinant large mechanosensitive ion channel of Escherichia coli (MscL) that yielded two-dimensional crystals. For that(More)