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Identification of Salmonella typhimurium genes responsible for interference with peptide presentation on MHC class I molecules: Δyej Salmonella mutants induce superior CD8+ T‐cell responses
Salmonella‐derived epitopes are presented on MHC molecules by antigen‐presenting cells, and both CD4+ and CD8+ T cells participate in protective immunity to Salmonella. Therefore, mechanisms that… Expand
Reliable determination of transposon insertion site in prokaryotes by direct sequencing.
- U. Qimron, Neta Madar, Rivi Ascarelli-Goell, M. Elgrably-Weiss, S. Altuvia, A. Porgador
- Biology, Medicine
- Journal of microbiological methods
- 1 July 2003
We developed a method to identify the insertion sites of transposons in the chromosome of Salmonella using one step only. In this method, the Salmonella's genomic DNA is directly sequenced using a… Expand