Nellie Schipper

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In 73 CAP-1 treated stage III and IV ovarian cancers, the prognostic significance of morphometric features and cellular DNA content has been evaluated in comparison with histologic type, grade of differentiation and a number of clinical characteristics. Borderline tumours were excluded from the study. Median follow-up was 44 months, median survival time 36(More)
This retrospective study evaluates several prognostic factors in 63 patients with invasive ductal breast cancer. Special attention is paid to the additional prognostic value of cellular DNA content to the previously developed and evaluated quantitative features mitotic activity index (MAI) and multivariate morphometric prognostic index (MPI). Follow-up was(More)
Compared with conventional light microscopy (LM), confocal laser scan microscopy (CLSM) is characterized in part by increased light sensitivity and higher spatial resolution. These characteristics permit the detection and discrimination of minor changes in cells and tissues in a more specific, sensitive, and accurate way. Potential clinical applications(More)
Changes in intracellular drug localization accompany doxorubicin resistance in multidrug resistant tumor cells. The purpose of this study was to develop a method to quantify these changes and so detect different levels of resistance. Tumor cells were incubated with the fluorescent anthracycline doxorubicin (excitation at 480 nm; emission maximum at 560-590(More)
The added prognostic value of cellular DNA content compared with single and combined morphometric factors and classical parameters such as tumor size, nodal status, histologic grade and estrogen receptor (ER) content was investigated in 225 consecutive breast-cancer patients with long follow-up. Of all features investigated, the MPI (multivariate prognostic(More)
Post-mortem radiography of fetuses with skeletal dysplasia is essential for diagnostic classification. Interpretation of the radiographs should be based on the knowledge of morphology and dimensions of the normal skeleton in all stages of development. A retrospective post-mortem radiographic study is presented with measurements of the lengths of the long(More)
In a prospective blind evaluation of multiple colonic mucosal biopsy specimens, 45 clinically well defined patients with chronic inflammatory bowel disease (21 Crohn's disease and 24 ulcerative colitis) and 16 control subjects (seven normal subjects and nine patients with diverticular disease) were studied to identify reproducible histopathological features(More)
In 65 patients with primary invasive ductal breast carcinoma the relation between classic prognosticators describing the extent of disease (lymph node metastases and tumour size) and newer promising morphometric and DNA flow cytometric prognostic factors was studied. There was no relation between DNA ploidy, lymph node state, and tumour size. Tumours with a(More)
Cellular DNA content was determined by flow cytometry on routinely processed paraffin sections of 61 primary and untreated transitional cell carcinomas of the urinary bladder, and correlated with tumour grade and stage and clinical follow up. All 16 (25%) grade 1 carcinomas were diploid and all 11 (20%) grade 3 tumours were aneuploid. The 34 (55%) grade 2(More)
The paper describes an improved segmentation method to measure the percentages of epithelium and stroma in ovarian (tumor) tissue with automated image analysis and evaluates its prognostic value. In the image processing method, a blue-yellow image pair is recorded from standard paraffin sections and stained with pararosanilin Feulgen and naphthol yellow.(More)